Optical bunching of particles in a liquid flow

Awel, SalahDeutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci CFEL, Notkestr 85, D-22607 Hamburg, Germany. (författare)

High-speed liquid micro-jets are used to rapidly and repeatedly deliver protein microcrystals to focused and pulsed X-ray beams in the method of serial femtosecond crystallography. However, the current continuous flow of crystals is mismatched to the arrival of X-ray pulses, wasting vast amounts of an often rare and precious sample. Here, we introduce a method to address this problem by periodically trapping and releasing crystals in the liquid flow, creating locally concentrated crystal bunches, using an optical trap integrated in the microfluidic supply line. We experimentally demonstrate a 30-fold increase of particle concentration into 10 Hz bunches of 6.4 tm diameter polystyrene particles. Furthermore, using particle trajectory simulations, a comprehensive description of the optical bunching process and parameter space is presented. Adding this compact optofluidics device to existing injection systems would thereby dramatically reduce sample consumption and extend the application of serial crystallography to a greater range of protein crystal systems that cannot be produced in high abundance. Our approach is suitable for other microfluidic systems that require synchronous measurements of flowing objects. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Bohne, SvenHamburg Univ Technol, Eissendorfer Str 42, D-21073 Hamburg, Germany. (författare)
Ebrahimifard, RezaDeutsch Elektronen Synchrotron DESY, Notkestr 85, D-22607 Hamburg, Germany. (författare)
Trieu, Hoc Khiem (författare)
Bajt, SasaDeutsch Elektronen Synchrotron DESY, Notkestr 85, D-22607 Hamburg, Germany.;Hamburg Ctr Ultrafast Imaging, Luruper Chaussee 149, D-22761 Hamburg, Germany. (författare)
Chapman, HenryUppsala universitet,Kemisk och biomolekylär fysik,Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci CFEL, Notkestr 85, D-22607 Hamburg, Germany.;Hamburg Ctr Ultrafast Imaging, Luruper Chaussee 149, D-22761 Hamburg, Germany.;Univ Hamburg, Dept Phys, Luruper Chaussee 149, D-22761 Hamburg, Germany.;Uppsala Univ, Dept Phys & Astron, Mol & Condensed Matter Phys, Uppsala, Sweden.(Swepub:uu)hench675 (författare)
Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci CFEL, Notkestr 85, D-22607 Hamburg, Germany.Hamburg Univ Technol, Eissendorfer Str 42, D-21073 Hamburg, Germany. (creator_code:org_t)

Ingår i:Optics Express: Optical Society of America29:21, s. 34394-344101094-4087

Av författaren/redakt...: Awel, Salah; Bohne, Sven; Ebrahimifard, Re ...; Trieu, Hoc Khiem; Bajt, Sasa; Chapman, Henry

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