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Quantitation of sev...
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Johansson, AnnaNatl Board Forens Med, Dept Forens Genet & Forens Toxicol, Artillerigatan 12, S-58758 Linköping, Sweden.
(författare)
Quantitation of seven sedative and analgesic drugs in whole blood from intensive care patients using liquid chromatography mass spectrometry
- Artikel/kapitelEngelska2021
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Elsevier,2021
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LIBRIS-ID:oai:DiVA.org:uu-461142
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-461142URI
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https://doi.org/10.1016/j.toxac.2021.08.016DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-181655URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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Funding Agencies|Swedish National Board of Forensic Medicine
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We present the development and validation of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantification of clonidine, dexmedetomidine, fentanyl, ketamine, ketobemidone, midazolam and morphine in whole blood. These are drugs predominately used in intensive care units (ICUs) but they are also encountered in forensic investigations. The analytes were recovered from 0.25 g of blood by protein precipitation with a mixture of acetonitrile and ethanol. Separation was performed on a BEH phenyl column. Mobile phases consisted of 0.05% formic acid in 10 mM ammonium formate and 0.05% formic acid in methanol, respectively, and the flow rate was 600 mu L/min. The mass spectrometer was operated in positive electrospray ionization mode with multiple reaction monitoring. Validation included selectivity, qualitative matrix effects, calibration model, limit of detection, lower limit of quantification, within- and between-day accuracy and precision, process efficiency, dilution integrity, carry over and stability. Selectivity was high and no ion suppression or enhancementwas observed in the areas were the analytes eluted. Calibration curves were linear over arange of 0.25-50 ng/g for dexmedetomidine, 0.05-50 ng/g for fentanyl and 5.0-500 ng/g formorphine and quadratic over a range of 0.5-50 ng/g for clonidine, 50-5000 ng/g for ketamine, 5.0-500 ng/g for ketobemidone and midazolam. The method showed acceptable within- and betweenday accuracies and precisions. All analytes were stable in whole blood for three weeksat 4. C. Concentrations in patient samples ranged between 42-760 ng/g for midazolam (n = 15), 0.3-1.5 ng/g for dexmedetomidine (n = 13), 0.6-6.4 ng/g for clonidine (n = 13), 8-62 ng/g for morphine (n = 16), 5-19 ng/g for ketobemidone (n = 5), 0.07-3.1 ng/g for fentanyl (n = 43), and 562000 ng/g for ketamine (n = 10). We conclude that the method was successfully validatedand applied to ante-mortem and post-mortem blood samples from critically ill adult patientsin a general ICU.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Ahlner, JohanLinköpings universitet,Avdelningen för klinisk kemi och farmakologi,Medicinska fakulteten,Linköping Univ, Div Clin Chem & Pharmacol, Dept Biomed & Clin Sci, Linköping, Sweden.(Swepub:liu)johah59
(författare)
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Lennborn, UlricaUppsala universitet,Anestesiologi och intensivvård,Uppsala Univ, Sweden(Swepub:uu)ulral766
(författare)
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Nielsen, Elisabet I.,1973-Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Uppsala Univ, Sweden(Swepub:uu)elnie838
(författare)
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Sandler, HåkanUppsala universitet,Rättsmedicin,Natl Board Forens Med, Dept Forens Med, Uppsala, Sweden.,Natl Board Forens Med, Sweden; Uppsala Univ, Sweden(Swepub:uu)hakasand
(författare)
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Rubertsson, StenUppsala universitet,Anestesiologi och intensivvård,Uppsala Univ, Sweden(Swepub:uu)stenrube
(författare)
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Kronstrand, RobertLinköpings universitet,Avdelningen för klinisk kemi och farmakologi,Medicinska fakulteten,Natl Board Forens Med, Dept Forens Genet & Forens Toxicol, Artillerigatan 12, S-58758 Linköping, Sweden.;Linköping Univ, Div Clin Chem & Pharmacol, Dept Biomed & Clin Sci, Linköping, Sweden.(Swepub:liu)robkr41
(författare)
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Kugelberg, FredrikLinköpings universitet,Avdelningen för klinisk kemi och farmakologi,Medicinska fakulteten,Natl Board Forens Med, Dept Forens Genet & Forens Toxicol, Artillerigatan 12, S-58758 Linköping, Sweden.;Linköping Univ, Div Clin Chem & Pharmacol, Dept Biomed & Clin Sci, Linköping, Sweden.(Swepub:liu)freku57
(författare)
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Natl Board Forens Med, Dept Forens Genet & Forens Toxicol, Artillerigatan 12, S-58758 Linköping, Sweden.Avdelningen för klinisk kemi och farmakologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:TOXICOLOGIE ANALYTIQUE ET CLINIQUE: Elsevier33:4, s. 327-3372352-00782352-0086
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