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  • Handin, NiklasUppsala universitet,Institutionen för farmaci (author)

Conditions for maintenance of hepatocyte differentiation and function in 3D cultures

  • Article/chapterEnglish2021

Publisher, publication year, extent ...

  • Cell Press,2021
  • electronicrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-463531
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-463531URI
  • https://doi.org/10.1016/j.isci.2021.103235DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Spheroid cultures of primary human hepatocytes (PHH) are used in studies of hepatic drug metabolism and toxicity. The cultures are maintained under different cone-lions, with possible confounding results. We performed an in-depth analysis of the influence of various culture conditions to find the optimal conditions for the maintenance of an in vivo like phenotype. The formation, protein expression, and function of PHH spheroids were followed for three weeks in a high-throughput 384-well format. Medium composition affected spheroid histology, global proteome profile, drug metabolism and drug-induced toxicity. No epithelial-mesenchymel transition was observed. Media with fasting glucose and insulin levels gave spheroids with phenotypes closest to normal PHH. The most expensive medium resulted in PHH features most divergent from that of native PHH. Our results provide a protocol for culture of healthy PHH with maintained function a prerequisite for studies of hepatocyte homeostasis and more reproducible hepatocyte research.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Mickols, EvgeniyaUppsala universitet,Institutionen för farmaci(Swepub:uu)evgin966 (author)
  • Ölander, MagnusUppsala universitet,Institutionen för farmaci(Swepub:uu)magol221 (author)
  • Rudfeldt, JakobUppsala universitet,Cancerfarmakologi och beräkningsmedicin(Swepub:uu)jakru319 (author)
  • Blom, KristinUppsala universitet,Cancerfarmakologi och beräkningsmedicin,Klinisk kemi(Swepub:uu)kribl842 (author)
  • Nyberg, FridaUppsala universitet,Cancerfarmakologi och beräkningsmedicin(Swepub:uu)friny434 (author)
  • Senkowski, WojciechUppsala universitet,Cancerfarmakologi och beräkningsmedicin,Uppsala Univ, Dept Med Sci, Div Canc Pharmacol & Computat Med, Uppsala, Sweden.;Univ Copenhagen, Biotech Res & Innovat Ctr BRIC, DK-2200 Copenhagen N, Denmark.;Univ Copenhagen, Novo Nordisk Fdn Ctr, Stem Cell Biol DanStem, DK-2200 Copenhagen N, Denmark.(Swepub:uu)wojse115 (author)
  • Urdzik, JozefUppsala universitet,Gastrointestinalkirurgi(Swepub:uu)jozur974 (author)
  • Maturi, VarunUppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk biokemi och mikrobiologi,Institutionen för farmaci(Swepub:uu)varma720 (author)
  • Fryknäs, MårtenUppsala universitet,Cancerfarmakologi och beräkningsmedicin(Swepub:uu)mafry516 (author)
  • Artursson, PerUppsala universitet,Institutionen för farmaci,Science for Life Laboratory, SciLifeLab(Swepub:uu)perartur (author)
  • Uppsala universitetInstitutionen för farmaci (creator_code:org_t)

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  • In:iScience: Cell Press24:112589-0042

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