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Ancient DNA Methods...
Ancient DNA Methods Improve Forensic DNA Profiling of Korean War and World War II Unknowns
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- Zavala, Elena I. (författare)
- Max Planck Inst Evolutionary Anthropol, Dept Evolutionary Genet, D-04103 Leipzig, Germany.
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- Thomas, Jacqueline Tyler (författare)
- Dover Air Force Base, Armed Forces Med Examiner Syst Armed Forces DNA I, Dover, DE 19902 USA.;SNA Int, Contractor Supporting Armed Forces Med Examiner S, Alexandria, VA 22314 USA.
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- Andreaggi, Kimberly (författare)
- Uppsala universitet,Medicinsk genetik och genomik,Dover Air Force Base, Armed Forces Med Examiner Syst Armed Forces DNA I, Dover, DE 19902 USA.;SNA Int, Contractor Supporting Armed Forces Med Examiner S, Alexandria, VA 22314 USA.
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- Daniels-Higginbotham, Jennifer (författare)
- Dover Air Force Base, Armed Forces Med Examiner Syst Armed Forces DNA I, Dover, DE 19902 USA.;SNA Int, Contractor Supporting Armed Forces Med Examiner S, Alexandria, VA 22314 USA.
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- Meyers, Kerriann K. (författare)
- Dover Air Force Base, Armed Forces Med Examiner Syst Armed Forces DNA I, Dover, DE 19902 USA.;SNA Int, Contractor Supporting Armed Forces Med Examiner S, Alexandria, VA 22314 USA.
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- Barrit-Ross, Suzanne (författare)
- Dover Air Force Base, Armed Forces Med Examiner Syst Armed Forces DNA I, Dover, DE 19902 USA.
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- Aximu-Petri, Ayinuer (författare)
- Max Planck Inst Evolutionary Anthropol, Dept Evolutionary Genet, D-04103 Leipzig, Germany.
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- Richter, Julia (författare)
- Max Planck Inst Evolutionary Anthropol, Dept Evolutionary Genet, D-04103 Leipzig, Germany.
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- Nickel, Birgit (författare)
- Max Planck Inst Evolutionary Anthropol, Dept Evolutionary Genet, D-04103 Leipzig, Germany.
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- Berg, Gregory E. (författare)
- Hickam Air Force Base, Def Personnel Accounting Agcy, Cent Identificat Lab, Oahu, HI 96853 USA.
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- McMahon, Timothy P. (författare)
- Dover Air Force Base, Armed Forces Med Examiner Syst Armed Forces DNA I, Dover, DE 19902 USA.;SNA Int, Contractor Supporting Armed Forces Med Examiner S, Alexandria, VA 22314 USA.
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- Meyer, Matthias (författare)
- Max Planck Inst Evolutionary Anthropol, Dept Evolutionary Genet, D-04103 Leipzig, Germany.
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- Marshall, Charla (författare)
- Dover Air Force Base, Armed Forces Med Examiner Syst Armed Forces DNA I, Dover, DE 19902 USA.;SNA Int, Contractor Supporting Armed Forces Med Examiner S, Alexandria, VA 22314 USA.;Penn State Univ, Forens Sci Program, State Coll, PA 16802 USA.
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Max Planck Inst Evolutionary Anthropol, Dept Evolutionary Genet, D-04103 Leipzig, Germany Dover Air Force Base, Armed Forces Med Examiner Syst Armed Forces DNA I, Dover, DE 19902 USA.;SNA Int, Contractor Supporting Armed Forces Med Examiner S, Alexandria, VA 22314 USA. (creator_code:org_t)
- 2022-01-11
- 2022
- Engelska.
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Ingår i: Genes. - : MDPI. - 2073-4425. ; 13:1
- Relaterad länk:
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https://uu.diva-port... (primary) (Raw object)
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https://www.mdpi.com...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- The integration of massively parallel sequencing (MPS) technology into forensic casework has been of particular benefit to the identification of unknown military service members. However, highly degraded or chemically treated skeletal remains often fail to provide usable DNA profiles, even with sensitive mitochondrial (mt) DNA capture and MPS methods. In parallel, the ancient DNA field has developed workflows specifically for degraded DNA, resulting in the successful recovery of nuclear DNA and mtDNA from skeletal remains as well as sediment over 100,000 years old. In this study we use a set of disinterred skeletal remains from the Korean War and World War II to test if ancient DNA extraction and library preparation methods improve forensic DNA profiling. We identified an ancient DNA extraction protocol that resulted in the recovery of significantly more human mtDNA fragments than protocols previously used in casework. In addition, utilizing single-stranded rather than double-stranded library preparation resulted in increased attainment of reportable mtDNA profiles. This study emphasizes that the combination of ancient DNA extraction and library preparation methods evaluated here increases the success rate of DNA profiling, and likelihood of identifying historical remains.
Ämnesord
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Annan medicin och hälsovetenskap -- Rättsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Other Medical and Health Sciences -- Forensic Science (hsv//eng)
Nyckelord
- degraded DNA
- massively parallel sequencing (MPS)
- mitochondrial DNA
- forensic DNA profiling
- ancient DNA
- human identification
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- Av författaren/redakt...
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Zavala, Elena I.
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Thomas, Jacqueli ...
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Andreaggi, Kimbe ...
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Daniels-Higginbo ...
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Meyers, Kerriann ...
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Barrit-Ross, Suz ...
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Aximu-Petri, Ayi ...
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Richter, Julia
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Nickel, Birgit
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Berg, Gregory E.
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McMahon, Timothy ...
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Meyer, Matthias
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Marshall, Charla
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Genetik
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Annan medicin oc ...
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och Rättsmedicin
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Genes
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Uppsala universitet