Search: WFRF:(Skog Oskar Docent PhD 1981 ) >
Characterisation of...
Characterisation of enterovirus RNA detected in the pancreas and other specimens of live patients with newly diagnosed type 1 diabetes in the DiViD study
-
- Oikarinen, Sami (author)
- Tampere Univ, Fac Med & Hlth Technol, Tampere, Finland.;Univ Oslo, Oslo Univ Hosp, Dept HPB Surg, Intervent Ctr, Oslo, Norway.
-
- Krogvold, Lars (author)
- Oslo Univ Hosp, Dept Paediat, Oslo, Norway.;Univ Oslo, Fac Med, Oslo, Norway.
-
- Edwin, Bjorn (author)
- Oslo Univ Hosp, Dept Paediat, Oslo, Norway.;Univ Oslo, Oslo Univ Hosp, Dept HPB Surg, Intervent Ctr, Oslo, Norway.;Univ Oslo, Inst Clin Med, Oslo, Norway.
-
show more...
-
- Buanes, Trond (author)
- Oslo Univ Hosp, Dept Paediat, Oslo, Norway.;Univ Oslo, Inst Clin Med, Oslo, Norway.;Univ Oslo, Oslo Univ Hosp, Inst Clin Med, Dept Hepato Pancreato Biliary Surg, Oslo, Norway.;Univ Oslo, Oslo Univ Hosp, Div Canc, Surg & Transplantat, Oslo, Norway.
-
- Korsgren, Olle (author)
- Uppsala universitet,Klinisk immunologi,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk cellbiologi,Uppsala Univ, Sweden
-
- Laiho, Jutta E. (author)
- Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland
-
- Oikarinen, Maarit (author)
- Tampere Univ, Fac Med & Hlth Technol, Tampere, Finland.
-
- Ludvigsson, Johnny (author)
- Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten,Region Östergötland, H.K.H. Kronprinsessan Victorias barn- och ungdomssjukhus,Linköping Univ, Div Pediat, Dept Biomed & Clin Sci, Linköping, Sweden.
-
- Skog, Oskar, Docent, PhD, 1981- (author)
- Uppsala universitet,Klinisk immunologi,Uppsala Univ, Sweden
-
- Anagandula, Mahesh, 1982- (author)
- Uppsala universitet,Klinisk immunologi,Uppsala Univ, Sweden
-
- Frisk, Gun (author)
- Uppsala universitet,Institutionen för kvinnors och barns hälsa,Klinisk immunologi,Uppsala Univ, Sweden
-
- Hyoty, Heikki (author)
- Tampere Univ, Fac Med & Hlth Technol, Tampere, Finland.;Linköping Univ, Crown Princess Victoria Childrens Hosp, Linköping, Sweden.,Tampere Univ, Finland; Fimlab Laboratories, Pirkanmaa Hospital District, Tampere, Finland
-
- Dahl-Jorgensen, Knut (author)
- Oslo Univ Hosp, Dept Paediat, Oslo, Norway.;Univ Oslo, Fac Med, Oslo, Norway.;Pirkanmaa Hosp Dist, Fimlab Labs, Tampere, Finland.
-
show less...
-
Tampere Univ, Fac Med & Hlth Technol, Tampere, Finland;Univ Oslo, Oslo Univ Hosp, Dept HPB Surg, Intervent Ctr, Oslo, Norway. Oslo Univ Hosp, Dept Paediat, Oslo, Norway.;Univ Oslo, Fac Med, Oslo, Norway. (creator_code:org_t)
- 2021-08-14
- 2021
- English.
-
In: Diabetologia. - : Springer Nature. - 0012-186X .- 1432-0428. ; 64:11, s. 2491-2501
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://uu.diva-port... (primary) (Raw object)
-
https://link.springe...
-
https://liu.diva-por... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- Aims/hypothesis The Diabetes Virus Detection (DiViD) study is the first study to laparoscopically collect pancreatic tissue and purified pancreatic islets together with duodenal mucosa, serum, peripheral blood mononuclear cells (PBMCs) and stools from six live adult patients (age 24-35 years) with newly diagnosed type 1 diabetes. The presence of enterovirus (EV) in the pancreatic islets of these patients has previously been reported. Methods In the present study we used reverse transcription quantitative real-time PCR (RT-qPCR) and sequencing to characterise EV genomes present in different tissues to understand the nature of infection in these individuals. Results All six patients were found to be EV-positive by RT-qPCR in at least one of the tested sample types. Four patients were EV-positive in purified islet culture medium, three in PBMCs, one in duodenal biopsy and two in stool, while serum was EVnegative in all individuals. Sequencing the 5' untranslated region of these EVs suggested that all but one belonged to enterovirus B species. One patient was EV-positive in all these sample types except for serum. Sequence analysis revealed that the virus strain present in the isolated islets of this patient was different from the strain found in other sample types. None of the islet-resident viruses could be isolated using EV-permissive cell lines. Conclusions/interpretation EV RNA can be frequently detected in various tissues of patients with type 1 diabetes. At least in some patients, the EV strain in the pancreatic islets may represent a slowly replicating persisting virus.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- Enterovirus
- Laparoscopy
- Organs
- Pancreas
- Persistent infection
- RT-qPCR
- Sequence
- Type 1 diabetes
- Viral RNA
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Oikarinen, Sami
-
Krogvold, Lars
-
Edwin, Bjorn
-
Buanes, Trond
-
Korsgren, Olle
-
Laiho, Jutta E.
-
show more...
-
Oikarinen, Maari ...
-
Ludvigsson, John ...
-
Skog, Oskar, Doc ...
-
Anagandula, Mahe ...
-
Frisk, Gun
-
Hyoty, Heikki
-
Dahl-Jorgensen, ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Endocrinology an ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Microbiology in ...
- Articles in the publication
-
Diabetologia
- By the university
-
Uppsala University
-
Linköping University