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Molecular Detection of Colistin Resistance mcr-1 Gene in Multidrug-Resistant Escherichia coli Isolated from Chicken
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- Uddin, Md Bashir (author)
- Sylhet Agr Univ, Dept Med, Sylhet 3100, Bangladesh.
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- Alam, Mohammad Nurul (author)
- Sylhet Agr Univ, Dept Med, Sylhet 3100, Bangladesh.
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- Hasan, Mahmudul (author)
- Sylhet Agr Univ, Dept Pharmaceut & Ind Biotechnol, Sylhet 3100, Bangladesh.
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- Hossain, S. M. Bayejed (author)
- Sylhet Agr Univ, Dept Med, Sylhet 3100, Bangladesh.
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- Debnath, Mita (author)
- Kazi Farms Poultry Lab, Gazipur 1700, Bangladesh.
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- Begum, Ruhena (author)
- Bangladesh Livestock Res Inst BLRI, Savar 1341, Bangladesh.
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- Samad, Mohammed A. (author)
- Bangladesh Livestock Res Inst BLRI, Savar 1341, Bangladesh.
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- Hoque, Syeda Farjana (author)
- Sylhet Agr Univ, Dept Pharmaceut & Ind Biotechnol, Sylhet 3100, Bangladesh.
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- Chowdhury, Md. Shahidur Rahman (author)
- Sylhet Agr Univ, Dept Med, Sylhet 3100, Bangladesh.
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- Rahman, Md. Mahfujur (author)
- Sylhet Agr Univ, Dept Med, Sylhet 3100, Bangladesh.
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- Hossain, Md. Mukter (author)
- Sylhet Agr Univ, Dept Med, Sylhet 3100, Bangladesh.
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- Hassan, Mohammad Mahmudul (author)
- Chattogram Vet & Anim Sci Univ, Dept Physiol Biochem & Pharmacol, Chattogram 4225, Bangladesh.
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- Lundkvist, Åke (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Klinisk mikrobiologi
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- Järhult, Josef D., 1975- (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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- El Zowalaty, Mohamed E. (author)
- Uppsala Univ, Zoonosis Sci Ctr, Dept Med Biochem & Microbiol, SE-75123 Uppsala, Sweden.
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- Ahmed, Syed Sayeem Uddin (author)
- Sylhet Agr Univ, Dept Epidmiol & Publ Hlth, Sylhet 3100, Bangladesh.
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Sylhet Agr Univ, Dept Med, Sylhet 3100, Bangladesh Sylhet Agr Univ, Dept Pharmaceut & Ind Biotechnol, Sylhet 3100, Bangladesh. (creator_code:org_t)
- 2022-01-13
- 2022
- English.
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In: Antibiotics. - : MDPI AG. - 2079-6382. ; 11:1
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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https://doi.org/10.3...
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Abstract
Subject headings
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- Zoonotic and antimicrobial-resistant Escherichia coli (hereafter, E. coli) is a global public health threat which can lead to detrimental effects on human health. Here, we aim to investigate the antimicrobial resistance and the presence of mcr-1 gene in E. coli isolated from chicken feces. Ninety-four E. coli isolates were obtained from samples collected from different locations in Bangladesh, and the isolates were identified using conventional microbiological tests. Phenotypic disk diffusion tests using 20 antimicrobial agents were performed according to CLSI-EUCAST guidelines, and minimum inhibitory concentrations (MICs) were determined for a subset of samples. E. coli isolates showed high resistance to colistin (88.30%), ciprofloxacin (77.66%), trimethoprim/sulfamethoxazole (76.60%), tigecycline (75.53%), and enrofloxacin (71.28%). Additionally, the pathotype eaeA gene was confirmed in ten randomly selected E. coli isolates using primer-specific polymerase chain reaction (PCR). The presence of mcr-1 gene was confirmed using PCR and sequencing analysis in six out of ten E. coli isolates. Furthermore, sequencing and phylogenetic analyses revealed a similarity between the catalytic domain of Neisseria meningitidis lipooligosaccharide phosphoethanolamine transferase A (LptA) and MCR proteins, indicating that the six tested isolates were colistin resistant. Finally, the findings of the present study showed that E. coli isolated from chicken harbored mcr-1 gene, and multidrug and colistin resistance. These findings accentuate the need to implement strict measures to limit the imprudent use of antibiotics, particularly colistin, in agriculture and poultry farms.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Keyword
- Escherichia coli
- antimicrobial resistance
- PCR
- mcr-1 gene
- poultry
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Uddin, Md Bashir
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Alam, Mohammad N ...
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Hasan, Mahmudul
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Hossain, S. M. B ...
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Debnath, Mita
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Begum, Ruhena
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Samad, Mohammed ...
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Hoque, Syeda Far ...
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Chowdhury, Md. S ...
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Rahman, Md. Mahf ...
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Hossain, Md. Muk ...
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Hassan, Mohammad ...
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Lundkvist, Åke
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Järhult, Josef D ...
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El Zowalaty, Moh ...
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Ahmed, Syed Saye ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Infectious Medic ...
- Articles in the publication
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Antibiotics
- By the university
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Uppsala University