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AGPAT1 as a Novel C...
AGPAT1 as a Novel Colonic Biomarker for Discriminating Between Ulcerative Colitis With and Without Primary Sclerosing Cholangitis
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- Vessby, Johan, 1972- (författare)
- Uppsala universitet,Gastroenterologi/hepatologi
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- Wisniewski, Jacek R. (författare)
- Max Planck Inst Biochem, Dept Prote & Signal Transduct, Biochem Prote Grp, Martinsried, Germany.
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- Lindskog, Cecilia (författare)
- Uppsala universitet,Klinisk och experimentell patologi
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- Eriksson, Niclas, 1978- (författare)
- Uppsala universitet,Uppsala kliniska forskningscentrum (UCR)
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- Gabrysch, Katja (författare)
- Uppsala universitet,Uppsala kliniska forskningscentrum (UCR)
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- Zettl, Katharina (författare)
- Max Planck Inst Biochem, Dept Prote & Signal Transduct, Biochem Prote Grp, Martinsried, Germany.
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- Wanders, Alkwin (författare)
- Aalborg Univ Hosp, Dept Pathol, Aalborg, Denmark.
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- Carlson, Marie, 1957- (författare)
- Uppsala universitet,Gastroenterologi/hepatologi,Uppsala Univ, Gastroenterol Res Grp, Dept Med Sci, Uppsala, Sweden.
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- Rorsman, Fredrik, Docent, 1960- (författare)
- Uppsala universitet,Gastroenterologi/hepatologi,Uppsala Univ, Gastroenterol Res Grp, Dept Med Sci, Uppsala, Sweden.
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- Åberg, Mikael (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Klinisk kemi
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(creator_code:org_t)
- 2022-05
- 2022
- Engelska.
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Ingår i: Clinical and Translational Gastroenterology. - : Ovid Technologies (Wolters Kluwer Health). - 2155-384X. ; 13:5
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- INTRODUCTION: Ulcerative colitis (UC) associated with primary sclerosing cholangitis (PSC-UC) is considered a unique inflammatory bowel disease (IBD) entity. PSC diagnosis in an IBD individual entails a significantly higher risk of gastrointestinal cancer; however, biomarkers for identifying patients with UC at risk for PSC are lacking. We, therefore, performed a thorough PSC-UC biomarker study, starting from archived colonic tissue.METHODS: Proteins were extracted out of formalin-fixed paraffin-embedded proximal colon samples from PSC-UC (n = 9), UC (n = 7), and healthy controls (n = 7). Patients with IBD were in clinical and histological remission, and all patients with UC had a history of pancolitis. Samples were processed by the multienzyme digestion FASP and subsequently analyzed by liquid chromatography-tandem mass spectrometry. Candidate proteins were replicated in an independent cohort (n: PSC-UC = 16 and UC = 21) and further validated by immunohistochemistry.RESULTS: In the discovery step, 7,279 unique proteins were detected. The top 5 most differentiating proteins (PSC-UC vs UC) based on linear regression analysis were selected for replication. Of these, 1-acetylglycerol-3-phosphate O-acyltransferase 1 (AGPAT1) was verified as higher in PSC-UC than UC (P = 0.009) in the replication cohort. A difference on the group level was also confirmed by immunohistochemistry, showing more intense AGPAT1 staining in patients with PSC-UC compared with UC.DISCUSSION: We present AGPAT1 as a potential colonic biomarker for differentiating PSC-UC from UC. Our findings have possible implication for future PSC-IBD diagnostics and surveillance.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Gastroenterologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)
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- ref (ämneskategori)
- art (ämneskategori)
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Vessby, Johan, 1 ...
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Wisniewski, Jace ...
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Lindskog, Cecili ...
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Eriksson, Niclas ...
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Gabrysch, Katja
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Zettl, Katharina
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visa fler...
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Wanders, Alkwin
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Carlson, Marie, ...
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Rorsman, Fredrik ...
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Åberg, Mikael
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