A microfluidic-based approach to investigate the inflammatory response of macrophages to pristine and drug-loaded nanostructured hydroxyapatite

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Carter, Sarah-Sophia,1994-Uppsala universitet,Institutionen för materialvetenskap,Science for Life Laboratory, SciLifeLab,EMBLA (författare)

A microfluidic-based approach to investigate the inflammatory response of macrophages to pristine and drug-loaded nanostructured hydroxyapatite

Artikel/kapitelEngelska2022

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Elsevier,2022
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LIBRIS-ID:oai:DiVA.org:uu-480694
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-480694URI
https://doi.org/10.1016/j.mtbio.2022.100351DOI

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Språk:engelska
Sammanfattning på:engelska

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Ämneskategori:art swepub-publicationtype

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The in vitro biological characterization of biomaterials is largely based on static cell cultures. However, for highly reactive biomaterials such as calcium-deficient hydroxyapatite (CDHA), this static environment has limitations. Drastic alterations in the ionic composition of the cell culture medium can negatively affect cell behavior, which can lead to misleading results or data that is difficult to interpret. This challenge could be addressed by a microfluidics-based approach (i.e. on-chip), which offers the opportunity to provide a continuous flow of cell culture medium and a potentially more physiologically relevant microenvironment. The aim of this work was to explore microfluidic technology for its potential to characterize CDHA, particularly in the context of inflammation. Two different CDHA substrates (chemically identical, but varying in microstructure) were integrated on-chip and subsequently evaluated. We demonstrated that the on-chip environment can avoid drastic ionic alterations and increase protein sorption, which was reflected in cell studies with RAW 264.7 macrophages. The cells grown on-chip showed a high cell viability and enhanced proliferation compared to cells maintained under static conditions. Whereas no clear differences in the secretion of tumor necrosis factor alpha (TNF-α) were found, variations in cell morphology suggested a more anti-inflammatory environment on-chip. In the second part of this study, the CDHA substrates were loaded with the drug Trolox. We showed that it is possible to characterize drug release on-chip and moreover demonstrated that Trolox affects the TNF-α secretion and morphology of RAW 264.7 cells. Overall, these results highlight the potential of microfluidics to evaluate (bioactive) biomaterials, both in pristine form and when drug-loaded. This is of particular interest for the latter case, as it allows the biological characterization and assessment of drug release to take place under the same dynamic in vitro environment.

Ämnesord och genrebeteckningar

NATURVETENSKAP Biologi Biofysik hsv//swe
NATURAL SCIENCES Biological Sciences Biophysics hsv//eng
Biomaterial
Calcium phosphate cement
Drug release
In vitro
Macrophage
On-chip
Engineering Science with specialization in Biomedical Engineering
Teknisk fysik med inriktning mot medicinsk teknik

Biuppslag (personer, institutioner, konferenser, titlar ...)

Atif, Abdul Raouf,1996-Uppsala universitet,Institutionen för materialvetenskap,Science for Life Laboratory, SciLifeLab,EMBLA(Swepub:uu)abdat742 (författare)
Diez-Escudero, AnnaUppsala universitet,Ortopedi,Ortholab(Swepub:uu)anndi833 (författare)
Grape, MajaUppsala universitet,Institutionen för materialvetenskap,Science for Life Laboratory, SciLifeLab,EMBLA(Swepub:uu)majgr178 (författare)
Ginebra, Maria-PauBiomaterials, Biomechanics and Tissue Engineering Group, Departament de Ciència i Enginyeria de Materials, Universitat Politècnica de Catalunya (UPC), 08930, Barcelona, Spain.;Barcelona Research Center in Multiscale Science and Engineering, Universitat Politècnica de Catalunya, 08930, Barcelona, Spain.;Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology (BIST), Baldiri Reixac 10-12, 08028, Barcelona, Spain. (författare)
Tenje, MariaUppsala universitet,Institutionen för materialvetenskap,Science for Life Laboratory, SciLifeLab,EMBLA(Swepub:uu)marte323 (författare)
Mestres, Gemma,1984-Uppsala universitet,Institutionen för materialvetenskap,Science for Life Laboratory, SciLifeLab,EMBLA(Swepub:uu)gemme924 (författare)
Uppsala universitetInstitutionen för materialvetenskap (creator_code:org_t)

Sammanhörande titlar

Ingår i:Materials Today Bio: Elsevier162590-0064

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