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The Influence of Pl...
The Influence of Plant Stress Hormones and Biotic Elicitors on Cyclotide Production in Viola uliginosa Cell Suspension Cultures
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- Slazak, Blazej (författare)
- Uppsala universitet,Farmakognosi,Institutionen för farmaceutisk biovetenskap,Polish Acad Sci, W Szafer Inst Bot, 46 Lubicz, PL-31512 Krakow, Poland.
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- Jedrzejska, Aleksandra (författare)
- Polish Acad Sci, W Szafer Inst Bot, 46 Lubicz, PL-31512 Krakow, Poland.
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- Badyra, Bogna (författare)
- Polish Acad Sci, Nencki EMBL Ctr Excellence Neural Plast & Brain D, Lab Neurobiol, Nencki Inst Expt Biol, PL-02093 Warsaw, Poland.
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- Shariatgorji, Reza (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Science for Life Laboratory, SciLifeLab
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- Nilsson, Anna (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Science for Life Laboratory, SciLifeLab
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- Andrén, Per E., Professor, 1957- (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Science for Life Laboratory, SciLifeLab
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- Göransson, Ulf, 1970- (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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(creator_code:org_t)
- 2022-07-19
- 2022
- Engelska.
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Ingår i: PLANTS. - : MDPI. - 2223-7747. ; 11:14
- Relaterad länk:
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Cyclotides are macrocycle peptides produced by plants from several families, including Violaceae. These compounds have the potential for applications in medicine, bioengineering and crop protection thanks to their multiple biological activities. In most cases, cyclotides are extracted from plant material. Plant cell culture provides a viable and sustainable form of plant biomass production Cyclotides are host defense peptides. The aim of the current study was to test whether different plant stress hormones and biological elicitors have effects on cyclotide production in Viola uliginosa suspension cultures. Different concentrations of jasmonic acid (JA), salicylic acid (SA), abscisic acid (ABA) and neutralized pathogens were tested. The cyclotide production was assessed using MALDI-MS. Five major peptides produced by V. uliginosa cultures were chosen for analysis, of which one was sequenced de novo. The treatments had little influence on the suspension's growth, with the exception of 100 mu M SA, which enhanced the biomass increase, and 100 mu M ABA, which was toxic. Significant increases in the production of three cyclotides (viul M, cyO13 and cyO3) were observed in suspensions primed with JA (50 mu M, 100 mu M, 200 mu M) after 14 days of culturing. Biotic elicitors had no observable effect on cyclotide production. The current study indicates that some cyclotides in V. uliginosa are triggered in response to JA. The stress plant hormones can be used to enhance plant cell culture-based production systems.
Ämnesord
- NATURVETENSKAP -- Biologi -- Botanik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Botany (hsv//eng)
Nyckelord
- plant in vitro cultures
- cyclic peptides
- host-defense peptides (HDPs)
- medicinal peptides
- plant stress response
- MALDI-MS
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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