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Endocrine disruptin...
Endocrine disrupting chemicals interfere with decidualization of human primary endometrial stromal cells in vitro
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- Lavogina, Darja (författare)
- Univ Tartu, Inst Chem, Tartu, Estonia.;Competence Ctr Hlth Technol, Tartu, Estonia.
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- Visser, Nadja (författare)
- Uppsala universitet,Institutionen för kvinnors och barns hälsa
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- Samuel, Kulli (författare)
- Competence Ctr Hlth Technol, Tartu, Estonia.
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- Davey, Eva (författare)
- Uppsala universitet,Reproduktionsbiologi
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- Bjorvang, Richelle D. (författare)
- Karolinska Institutet
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- Hassan, Jasmin (författare)
- Karolinska Inst, Dept Clin Sci Intervent & Technol, Div Obstet & Gynecol, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.
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- Koponen, Jani (författare)
- Finnish Inst Hlth & Welf, Dept Hlth Secur, Kuopio, Finland.
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- Rantakokko, Panu (författare)
- Finnish Inst Hlth & Welf, Dept Hlth Secur, Kuopio, Finland.
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- Kiviranta, Hannu (författare)
- Finnish Inst Hlth & Welf, Dept Hlth Secur, Kuopio, Finland.
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- Rinken, Ago (författare)
- Univ Tartu, Inst Chem, Tartu, Estonia.
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- Olovsson, Matts, 1958- (författare)
- Uppsala universitet,Reproduktionsbiologi
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- Salumets, Andres (författare)
- Karolinska Institutet
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- Damdimopoulou, Pauliina (författare)
- Karolinska Institutet
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Univ Tartu, Inst Chem, Tartu, Estonia;Competence Ctr Hlth Technol, Tartu, Estonia. Institutionen för kvinnors och barns hälsa (creator_code:org_t)
- 2022-08-19
- 2022
- Engelska.
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Ingår i: Frontiers in Endocrinology. - : Frontiers Media S.A.. - 1664-2392. ; 13
- Relaterad länk:
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Multiple studies have shown associations between exposure to endocrine disrupting chemicals (EDCs) and reduced fertility in women. However, little is known about the target organs of chemical disruption of female fertility. Here, we focus on the hormone-sensitive uterine lining, the endometrium, as a potential target. Decidualization is the morphological and functional change that endometrial stromal cells undergo to support endometrial receptivity, which is crucial for successful implantation, placentation, and pregnancy. We investigated the effect of nine selected EDCs on primary human endometrial stromal cell decidualization in vitro. The cells were exposed to a decidualization-inducing mixture in the presence or absence of 1 mu M of nine different EDCs for nine days. Extent of decidualization was assessed by measuring the activity of cAMP dependent protein kinase, Rho-associated coiled-coil containing protein kinase, and protein kinase B in lysates using photoluminescent probes, and secretion of prolactin into the media by using ELISA. Decidualization-inducing mixture upregulated activity of protein kinases and prolactin secretion in cells derived from all women. Of the tested chemicals, dichlorodiphenyldichloroethylene (p,p'-DDE), hexachlorobenzene (HCB) and perfluorooctanesulfonic acid (PFOS) significantly reduced decidualization as judged by the kinase markers and prolactin secretion. In addition, bisphenol A (BPA) reduced prolactin secretion but did not significantly affect activity of the kinases. None of the EDCs was cytotoxic, based on the assessment of total protein content or activity of the viability marker casein kinase 2 in lysates. These results indicate that EDCs commonly present in the blood circulation of reproductive-aged women can reduce decidualization of human endometrial stromal cells in vitro. Future studies should focus on detailed hazard assessment to define possible risks of EDC exposure to endometrial dysfunction and implantation failure in women.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reproduktionsmedicin och gynekologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Obstetrics, Gynaecology and Reproductive Medicine (hsv//eng)
Nyckelord
- endocrine disrupting chemical
- phthalate
- persistent organic pollutants (POPs)
- human endometrium
- decidualization
- per- and polyfluorinated alkyl substances (PFASs)
- primary stromal cells
- infertility
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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- Av författaren/redakt...
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Lavogina, Darja
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Visser, Nadja
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Samuel, Kulli
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Davey, Eva
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Bjorvang, Richel ...
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Hassan, Jasmin
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visa fler...
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Koponen, Jani
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Rantakokko, Panu
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Kiviranta, Hannu
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Rinken, Ago
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Olovsson, Matts, ...
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Salumets, Andres
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Damdimopoulou, P ...
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Uppsala universitet
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Karolinska Institutet