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Combined nanometric...
Combined nanometric and phylogenetic analysis of unique endocytic compartments in Giardia lamblia sheds light on the evolution of endocytosis in Metamonada
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- Santos, Rui (author)
- Univ Zurich, Inst Parasitol, Winterthurerstr 266A, CH-8057 Zurich, Switzerland.;Univ Zurich, Inst Anat, Winterthurerstr 190, CH-8057 Zurich, Switzerland.
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- Astvaldsson, Asgeir, 1981- (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi,Natl Vet Inst, Dept Microbiol, S-75123 Uppsala, Sweden.
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- Pipaliya, Shweta, V (author)
- Univ Alberta, Dept Med, Div Infect Dis, Edmonton, AB, Canada.;Ecole Polytech Fed Lausanne, Sch Life Sci, Lausanne, Switzerland.;Swiss Inst Bioinformat, Lausanne, Switzerland.
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- Zumthor, Jon Paulin (author)
- Amt Lebensmittelsicherheit & Tiergesundheit Graub, Chur, Switzerland.
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- Dacks, Joel B. (author)
- Univ Alberta, Dept Med, Div Infect Dis, Edmonton, AB, Canada.;CAS, Biol Ctr, Inst Parasitol, Branisovska 31, Ceske Budejovice 37005, Czech Republic.
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- Svärd, Staffan (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
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- Hehl, Adrian B. (author)
- Univ Zurich, Inst Parasitol, Winterthurerstr 266A, CH-8057 Zurich, Switzerland.
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- Faso, Carmen (author)
- Univ Bern, Inst Cell Biol, Bern, Switzerland.;Univ Bern, Multidisciplinary Ctr Infect Dis, Vetsuisse, Bern, Switzerland.
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Univ Zurich, Inst Parasitol, Winterthurerstr 266A, CH-8057 Zurich, Switzerland;Univ Zurich, Inst Anat, Winterthurerstr 190, CH-8057 Zurich, Switzerland. Institutionen för cell- och molekylärbiologi (creator_code:org_t)
- 2022-09-21
- 2022
- English.
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In: BMC Biology. - : BioMed Central (BMC). - 1741-7007. ; 20:1
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Abstract
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- Background Giardia lamblia, a parasitic protist of the Metamonada supergroup, has evolved one of the most diverged endocytic compartment systems investigated so far. Peripheral endocytic compartments, currently known as peripheral vesicles or vacuoles (PVs), perform bulk uptake of fluid phase material which is then digested and sorted either to the cell cytosol or back to the extracellular space. Results Here, we present a quantitative morphological characterization of these organelles using volumetric electron microscopy and super-resolution microscopy (SRM). We defined a morphological classification for the heterogenous population of PVs and performed a comparative analysis of PVs and endosome-like organelles in representatives of phylogenetically related taxa, Spironucleus spp. and Tritrichomonas foetus. To investigate the as-yet insufficiently understood connection between PVs and clathrin assemblies in G. lamblia, we further performed an in-depth search for two key elements of the endocytic machinery, clathrin heavy chain (CHC) and clathrin light chain (CLC), across different lineages in Metamonada. Our data point to the loss of a bona fide CLC in the last Fornicata common ancestor (LFCA) with the emergence of a protein analogous to CLC (GlACLC) in the Giardia genus. Finally, the location of clathrin in the various compartments was quantified. Conclusions Taken together, this provides the first comprehensive nanometric view of Giardia's endocytic system architecture and sheds light on the evolution of GlACLC analogues in the Fornicata supergroup and, specific to Giardia, as a possible adaptation to the formation and maintenance of stable clathrin assemblies at PVs.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- Endocytosis
- Super-resolution microscopy (SRM)
- Volumetric electron microscopy
- Peripheral endocytic compartments (PECs)
- Convergent evolution
- Metamonada
- Giardia
- Tritrichomonas
- Spironucleus
- Peripheral vacuoles
Publication and Content Type
- ref (subject category)
- art (subject category)
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