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Interacting polymer...
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Zhang, TianjiNatl Inst Metrol, Div Chem & Analyt Sci, Beijing, Peoples R China.;Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing, Peoples R China.;Key Lab Chem Metrol & Applicat Nutr & Hlth State M, Beijing, Peoples R China.
(author)
Interacting polymer-modification enzymes in heparan sulfate biosynthesis
- Article/chapterEnglish2023
Publisher, publication year, extent ...
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Elsevier,2023
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-488350
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-488350URI
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https://doi.org/10.1016/j.carbpol.2022.120191DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Glucuronyl 5-epimerase (Hsepi) converts D-glucuronic acid (GlcA) into L-iduronic acid (IdoA) units, through a mechanism involving reversible abstraction of a proton at C5 of hexuronic acid residues. Incubations of a [4GlcA beta 1-4GlcNSO3 alpha 1-]n precursor substrate with recombinant enzymes in a D2O/H2O medium enabled an isotope exchange approach to the assessment of functional interactions of Hsepi with hexuronyl 2-O-sulfotrans-ferase (Hs2st) and glucosaminyl 6-O-sulfotransferase (Hs6st), both involved in the final polymer-modification steps. Enzyme complexes were supported by computational modeling and homogeneous time resolved fluores-cence. GlcA and IdoA D/H ratios related to product composition revealed kinetic isotope effects that were interpreted in terms of efficiency of the coupled epimerase and sulfotransferase reactions. Evidence for a func-tional Hsepi/Hs6st complex was provided by selective incorporation of D atoms into GlcA units adjacent to 6-O -sulfated glucosamine residues. The inability to achieve simultaneous 2-O-and 6-O-sulfation in vitro supported topologically separated reactions in the cell. These findings provide novel insight into the roles of enzyme in-teractions in heparan sulfate biosynthesis.
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Yu, MingjiaBeijing Inst Technol, Sch Chem & Chem Engn, Beijing, Peoples R China.;Beijing Univ Chem Technol, Beijing Adv Innovat Ctr Soft Matter Sci & Engn, Beijing, Peoples R China.
(author)
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Li, HonglianUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi(Swepub:uu)honli111
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Maccarana, MarcoUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi(Swepub:uu)marma713
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Zhang, WeiNatl Inst Metrol, Div Chem & Analyt Sci, Beijing, Peoples R China.
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Shi, DelingShandong Univ, Natl Glycoengn Res Ctr, Jinan, Peoples R China.
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Kan, YingNatl Inst Metrol, Div Chem & Analyt Sci, Beijing, Peoples R China.;Key Lab Chem Metrol & Applicat Nutr & Hlth State M, Beijing, Peoples R China.
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Zhang, XiaoUppsala universitet,Forskargrupp Dan Larhammar(Swepub:uu)xizha255
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Chi, LianliShandong Univ, Natl Glycoengn Res Ctr, Jinan, Peoples R China.
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Lindahl, UlfUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi(Swepub:uu)uli08750
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Li, HongmeiNatl Inst Metrol, Div Chem & Analyt Sci, Beijing, Peoples R China.;Key Lab Chem Metrol & Applicat Nutr & Hlth State M, Beijing, Peoples R China.
(author)
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Li, Jin-PingUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Beijing Univ Chem Technol, Beijing Adv Innovat Ctr Soft Matter Sci & Engn, Beijing, Peoples R China.(Swepub:uu)jinpili
(author)
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Tan, TianweiBeijing Univ Chem Technol, Coll Life Sci & Technol, Beijing, Peoples R China.
(author)
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Natl Inst Metrol, Div Chem & Analyt Sci, Beijing, Peoples R China.;Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing, Peoples R China.;Key Lab Chem Metrol & Applicat Nutr & Hlth State M, Beijing, Peoples R China.Beijing Inst Technol, Sch Chem & Chem Engn, Beijing, Peoples R China.;Beijing Univ Chem Technol, Beijing Adv Innovat Ctr Soft Matter Sci & Engn, Beijing, Peoples R China.
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Related titles
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In:Carbohydrate Polymers: Elsevier2990144-86171879-1344
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- By the author/editor
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Zhang, Tianji
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Yu, Mingjia
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Li, Honglian
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Maccarana, Marco
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Zhang, Wei
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Shi, Deling
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Kan, Ying
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Zhang, Xiao
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Chi, Lianli
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Lindahl, Ulf
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Li, Hongmei
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Li, Jin-Ping
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Tan, Tianwei
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Carbohydrate Pol ...
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Uppsala University