Sökning: WFRF:(Lanekoff Ingela 1975) >
A Direct Infusion P...
A Direct Infusion Probe for Rapid Metabolomics of Low-Volume Samples
-
- Marques, Cátia (författare)
- Uppsala universitet,Analytisk kemi
-
- Liu, Liangwen (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
-
- Duncan, Kyle D. (författare)
- Uppsala universitet,Analytisk kemi
-
visa fler...
-
- Lanekoff, Ingela, Assoc. Prof. 1975- (författare)
- Uppsala universitet,Analytisk kemi
-
visa färre...
-
(creator_code:org_t)
- 2022-09-07
- 2022
- Engelska.
-
Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 94:37, s. 12875-12883
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://uu.diva-port... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Targeted and nontargeted metabolomics has the potential to evaluate and detect global metabolite changes in biological systems. Direct infusion mass spectrometric analysis enables detection of all ionizable small molecules, thus simultaneously providing information on both metabolites and lipids in chemically complex samples. However, to unravel the heterogeneity of the metabolic status of cells in culture and tissue a low number of cells per sample should be analyzed with high sensitivity, which requires low sample volumes. Here, we present the design and characterization of the direct infusion probe, DIP. The DIP is simple to build and position directly in front of a mass spectrometer for rapid metabolomics of chemically complex biological samples using pneumatically assisted electrospray ionization at 1 mu L/min flow rate. The resulting data is acquired in a square wave profile with minimal carryover between samples that enhances throughput and enables several minutes of uniform MS signal from 5 mu L sample volumes. The DIP was applied to study the intracellular metabolism of insulin secreting INS-1 cells and the results show that exposure to 20 mM glucose for 15 min significantly alters the abundance of several small metabolites, amino acids, and lipids.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas