SwePub
Sök i LIBRIS databas

  Utökad sökning

WFRF:(Zhang Mingzhi)
 

Sökning: WFRF:(Zhang Mingzhi) > Super-enhancers con...

  • Zhang, JuqingNorthwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)

Super-enhancers conserved within placental mammals maintain stem cell pluripotency

  • Artikel/kapitelEngelska2022

Förlag, utgivningsår, omfång ...

  • 2022-09-26
  • Proceedings of the National Academy of Sciences (PNAS),2022
  • electronicrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:uu-493202
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-493202URI
  • https://doi.org/10.1073/pnas.2204716119DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Despite pluripotent stem cells sharing key transcription factors, their maintenance involves distinct genetic inputs. Emerging evidence suggests that super-enhancers (SEs) can function as master regulatory hubs to control cell identity and pluripotency in humans and mice. However, whether pluripotency-associated SEs share an evolutionary origin in mammals remains elusive. Here, we performed comprehensive comparative epigenomic and transcription factor binding analyses among pigs, humans, and mice to identify pluripotency-associated SEs. Like typical enhancers, SEs displayed rapid evolu-tion in mammals. We showed that BRD4 is an essential and conserved activator for mammalian pluripotency-associated SEs. Comparative motif enrichment analysis revealed 30 shared transcription factor binding motifs among the three species. The majority of transcriptional factors that bind to identified motifs are known regulators associated with pluripotency. Further, we discovered three pluripotency-associated SEs (SE-SOX2, SE-PIM1, and SE-FGFR1) that displayed remarkable conservation in pla-cental mammals and were sufficient to drive reporter gene expression in a pluripotency-dependent manner. Disruption of these conserved SEs through the CRISPR-Cas9 approach severely impaired stem cell pluripotency. Our study provides insights into the understanding of conserved regulatory mechanisms underlying the maintenance of plu-ripotency as well as species-specific modulation of the pluripotency-associated regula-tory networks in mammals.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Zhou, YaqiNorthwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Yue, WeiNorthwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Zhu, ZhenshuoNorthwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Wu, XiaolongNorthwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Yu, ShuaiNorthwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Shen, QiaoyanNorthwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Pan, QinNorthwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Xu, WenjingNorthwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Zhang, RuiNorthwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Wu, XiaojieNorthwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Li, XinmeiNorthwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Li, YayuNorthwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Li, YunxiangNorthwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Wang, YuNorthwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Peng, ShaNorthwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Zhang, ShiqiangNorthwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Lei, AnminNorthwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Ding, XinbaoCornell Univ, Dept Biomed Sci, Coll Vet Med, Ithaca, NY 14853 USA. (författare)
  • Yang, FanNorthwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Chen, XingqiUppsala universitet,Molekylära verktyg och funktionsgenomik(Swepub:uu)xinch196 (författare)
  • Li, NaNorthwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Liao, MingzhiNorthwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Warg, WeiNatl Inst Biol Sci, Beijing 102206, Peoples R China.;Tsinghua Univ, Tsinghua Inst Multidisciplinary Biomed Res, Beijing 102206, Peoples R China. (författare)
  • Hua, JinlianNorthwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China. (författare)
  • Northwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China.Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China. (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Proceedings of the National Academy of Sciences of the United States of America: Proceedings of the National Academy of Sciences (PNAS)119:400027-84241091-6490

Internetlänk

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy