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Functional reconstitution of a bacterial CO2 concentrating mechanism in Escherichia coli

Flamholz, Avi I (författare)
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States
Dugan, Eli (författare)
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States
Blikstad, Cecilia (författare)
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States
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Gleizer, Shmuel (författare)
Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel
Ben-Nissan, Roee (författare)
Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel
Amram, Shira (författare)
Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel
Antonovsky, Niv (författare)
Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel
Ravishankar, Sumedha (författare)
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States
Noor, Elad (författare)
Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel
Bar-Even, Arren (författare)
Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany
Milo, Ron (författare)
Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel
Savage, David F (författare)
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States
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 (creator_code:org_t)
2020-10-21
2020
Engelska.
Ingår i: eLIFE. - eLife : eLife Sciences Publications, Ltd. - 2050-084X. ; 9
Relaterad länk:
https://doi.org/10.7...
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https://urn.kb.se/re...
https://doi.org/10.7...
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  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • Many photosynthetic organisms employ a CO2 concentrating mechanism (CCM) to increase the rate of CO2 fixation via the Calvin cycle. CCMs catalyze ≈50% of global photosynthesis, yet it remains unclear which genes and proteins are required to produce this complex adaptation. We describe the construction of a functional CCM in a non-native host, achieved by expressing genes from an autotrophic bacterium in an Escherichia coli strain engineered to depend on rubisco carboxylation for growth. Expression of 20 CCM genes enabled E. coli to grow by fixing CO2 from ambient air into biomass, with growth in ambient air depending on the components of the CCM. Bacterial CCMs are therefore genetically compact and readily transplanted, rationalizing their presence in diverse bacteria. Reconstitution enabled genetic experiments refining our understanding of the CCM, thereby laying the groundwork for deeper study and engineering of the cell biology supporting CO2 assimilation in diverse organisms.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

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