SwePub
Sök i LIBRIS databas

  Utökad sökning

WFRF:(Milo Shira)
 

Sökning: WFRF:(Milo Shira) > Functional reconsti...

  • Flamholz, Avi IDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States (författare)

Functional reconstitution of a bacterial CO2 concentrating mechanism in Escherichia coli

  • Artikel/kapitelEngelska2020

Förlag, utgivningsår, omfång ...

  • 2020-10-21
  • eLife :eLife Sciences Publications, Ltd,2020
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:uu-493302
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-493302URI
  • https://doi.org/10.7554/elife.59882DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Many photosynthetic organisms employ a CO2 concentrating mechanism (CCM) to increase the rate of CO2 fixation via the Calvin cycle. CCMs catalyze ≈50% of global photosynthesis, yet it remains unclear which genes and proteins are required to produce this complex adaptation. We describe the construction of a functional CCM in a non-native host, achieved by expressing genes from an autotrophic bacterium in an Escherichia coli strain engineered to depend on rubisco carboxylation for growth. Expression of 20 CCM genes enabled E. coli to grow by fixing CO2 from ambient air into biomass, with growth in ambient air depending on the components of the CCM. Bacterial CCMs are therefore genetically compact and readily transplanted, rationalizing their presence in diverse bacteria. Reconstitution enabled genetic experiments refining our understanding of the CCM, thereby laying the groundwork for deeper study and engineering of the cell biology supporting CO2 assimilation in diverse organisms.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Dugan, EliDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States (författare)
  • Blikstad, CeciliaDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States (författare)
  • Gleizer, ShmuelDepartment of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel (författare)
  • Ben-Nissan, RoeeDepartment of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel (författare)
  • Amram, ShiraDepartment of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel (författare)
  • Antonovsky, NivDepartment of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel (författare)
  • Ravishankar, SumedhaDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States (författare)
  • Noor, EladDepartment of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel (författare)
  • Bar-Even, ArrenMax Planck Institute of Molecular Plant Physiology, Potsdam, Germany (författare)
  • Milo, RonDepartment of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel (författare)
  • Savage, David FDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States (författare)
  • Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:eLIFEeLife : eLife Sciences Publications, Ltd92050-084X

Internetlänk

Hitta via bibliotek

  • eLIFE (Sök värdpublikationen i LIBRIS)

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy