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Maximizing Laborato...
Maximizing Laboratory Production of Aflatoxins and Fumonisins for Use in Experimental Animal Feeds
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- Ochieng, Phillis E. E. (author)
- Univ Liege, Fac Vet Med, Dept Food Sci, Lab Food Anal, B-4000 Liege, Belgium.;Univ Ghent, Fac Vet Med, Dept Pathobiol Pharmacol & Zool Med, B-9820 Merelbeke, Belgium.
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- Kemboi, David C. C. (author)
- Univ Ghent, Fac Vet Med, Dept Pathobiol Pharmacol & Zool Med, B-9820 Merelbeke, Belgium.;Chuka Univ, Dept Anim Sci, POB 109-00625, Chuka, Kenya.
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- Scippo, Marie-Louise (author)
- Univ Liege, Fac Vet Med, Dept Food Sci, Lab Food Anal, B-4000 Liege, Belgium.
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- Gathumbi, James K. K. (author)
- Univ Nairobi, Fac Vet Med, Dept Vet Pathol Microbiol & Parasitol, POB 29053-00100, Nairobi, Kenya.
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- Kangethe, Erastus (author)
- POB 34405-00100, Nairobi, Kenya.
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- Doupovec, Barbara (author)
- DSM BIOMIN Res Ctr, A-3430 Tulln, Austria.
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- Croubels, Siska (author)
- Univ Ghent, Fac Vet Med, Dept Pathobiol Pharmacol & Zool Med, B-9820 Merelbeke, Belgium.
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- Lindahl, Johanna (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Int Livestock Res Inst ILRI, Dept Biosci, POB 30709-00100, Nairobi, Kenya.;Swedish Univ Agr Sci, Dept Clin Sci, S-7054 Uppsala, Sweden.,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences,International Livestock Research Institute (ILRI),Uppsala University
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- Antonissen, Gunther (author)
- Univ Ghent, Fac Vet Med, Dept Pathobiol Pharmacol & Zool Med, B-9820 Merelbeke, Belgium.;Univ Ghent, Fac Vet Med, Chair Poultry Hlth Sci, B-9820 Merelbeke, Belgium.
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- Okoth, Sheila (author)
- Univ Nairobi, Fac Sci & Technol, Dept Biol, POB 30197-00100, Nairobi, Kenya.
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Univ Liege, Fac Vet Med, Dept Food Sci, Lab Food Anal, B-4000 Liege, Belgium;Univ Ghent, Fac Vet Med, Dept Pathobiol Pharmacol & Zool Med, B-9820 Merelbeke, Belgium. Univ Ghent, Fac Vet Med, Dept Pathobiol Pharmacol & Zool Med, B-9820 Merelbeke, Belgium.;Chuka Univ, Dept Anim Sci, POB 109-00625, Chuka, Kenya. (creator_code:org_t)
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- 2022-11-30
- 2022
- English.
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In: Microorganisms. - : MDPI. - 2076-2607. ; 10:12
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Abstract
Subject headings
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- Warm and humid climatic conditions coupled with poor agricultural practices in sub-Saharan Africa favor the contamination of food and feed by Aspergillus flavus and Fusarium verticillioides fungi, which subsequently may produce aflatoxins (AFs) and fumonisins (FBs), respectively. The growth of fungi and the production of mycotoxins are influenced by physical (temperature, pH, water activity, light and aeration), nutritional, and biological factors. This study aimed at optimizing the conditions for the laboratory production of large quantities of AFs and FBs for use in the animal experiments. A. flavus and F. verticillioides strains, previously isolated from maize in Kenya, were used. Levels of AFB1 and total FBs (FB1, FB2, and FB3) in different growth substrates were screened using ELISA methods. Maize kernels inoculated with three different strains of A. flavus simultaneously and incubated at 29 degrees C for 21 days had the highest AFB1 level of 12,550 +/- 3397 mu g/kg of substrate. The highest level of total FBs (386,533 +/- 153,302 mu g/kg of substrate) was detected in cracked maize inoculated with three different strains of F. verticillioides and incubated for 21 days at temperatures of 22-25 degrees C in a growth chamber fitted with yellow light. These two methods are recommended for the mass production of AFB1 and FBs for animal feeding trials.
Subject headings
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Livsmedelsvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Food Science (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- Aspergillus flavus
- Fusarium verticillioides
- aflatoxins production
- fumonisin production
- mycotoxins
- food safety
- feed safety
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Ochieng, Phillis ...
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Kemboi, David C. ...
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Scippo, Marie-Lo ...
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Gathumbi, James ...
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Kangethe, Erastu ...
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Doupovec, Barbar ...
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Croubels, Siska
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Lindahl, Johanna
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Antonissen, Gunt ...
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Okoth, Sheila
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- About the subject
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- AGRICULTURAL SCIENCES
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AGRICULTURAL SCI ...
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and Agriculture Fore ...
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and Food Science
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
- Articles in the publication
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Microorganisms
- By the university
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Uppsala University
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Swedish University of Agricultural Sciences