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High prevalence of ...
High prevalence of Escherichia coli co-harboring conjugative plasmids with colistin- and carbapenem resistance genes in a wastewater treatment plant in China
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- Wang, Di (författare)
- Shandong Univ, Cheeloo Coll Med, Sch Publ Hlth, Dept Environm & Hlth, Jinan 250012, Shandong, Peoples R China.
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- Zou, Huiyun (författare)
- Shandong Univ, Cheeloo Coll Med, Sch Publ Hlth, Dept Environm & Hlth, Jinan 250012, Shandong, Peoples R China.
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- Zhao, Ling (författare)
- Shandong Univ, Cheeloo Coll Med, Sch Publ Hlth, Dept Environm & Hlth, Jinan 250012, Shandong, Peoples R China.
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- Li, Qi (författare)
- Shandong Univ, Cheeloo Coll Med, Sch Publ Hlth, Dept Environm & Hlth, Jinan 250012, Shandong, Peoples R China.
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- Meng, Min (författare)
- Shandong Univ, Cheeloo Coll Med, Sch Publ Hlth, Dept Environm & Hlth, Jinan 250012, Shandong, Peoples R China.
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- Li, Xuewen (författare)
- Shandong Univ, Cheeloo Coll Med, Sch Publ Hlth, Dept Environm & Hlth, Jinan 250012, Shandong, Peoples R China.;Shandong Univ, Sch Publ Hlth, Dept Environm & Hlth, Jinan 250012, Peoples R China.
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- Berglund, Björn (författare)
- Uppsala universitet,Mikrobiologi och immunologi
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Shandong Univ, Cheeloo Coll Med, Sch Publ Hlth, Dept Environm & Hlth, Jinan 250012, Shandong, Peoples R China Shandong Univ, Cheeloo Coll Med, Sch Publ Hlth, Dept Environm & Hlth, Jinan 250012, Shandong, Peoples R China.;Shandong Univ, Sch Publ Hlth, Dept Environm & Hlth, Jinan 250012, Peoples R China. (creator_code:org_t)
- Elsevier, 2023
- 2023
- Engelska.
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Ingår i: International journal of hygiene and environmental health. - : Elsevier. - 1438-4639 .- 1618-131X. ; 250
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Emergence and dissemination of resistance to last-resort antibiotics such as carbapenem and colistin is a growing, global health concern. Wastewater treatment plants (WWTPs) link human activities and the environment, can act as reservoirs and sources for emerging antibiotic resistance, and likely play a large role in antibiotic resistance transmission. The aim of this study was to investigate occurrence and characteristics of colistin-and carbapenem-resistant Escherichia coli (CCREC) in wastewater and sludge samples collected over a one-year period from different functional areas of an urban WWTP in Jinan city, Shandong, China. A total of 8 CCREC were isolated from 168 samples with selective agar and PCR, corresponding to high prevalence of 4.8%, co-harboring car-bapenem resistance genes (blaNDM) and colistin resistance gene (mcr-1) and subsequently whole-genome sequenced. Additionally, all isolates were multidrug-resistant by antimicrobial susceptibility testing and car-ried a variety of antibiotic resistance genes. Two isolates carrying virulence genes associated with avian path-ogenic E. coli were identified, one belonging to the high-risk clone O101:H9-ST167. Southern blotting was used to characterize CCREC isolates and plasmids carrying blaNDM-genes or mcr-1 could be transferred to a recipient strain E. coli J53 by in vitro conjugation assays. Resistance to other antibiotic classes were sporadically co-transferred to the transconjugant. Transposition of and mcr-1-carrying element from a transferable IncHI2-plasmid was observed among two CCREC clones isolated within 4 days of each other. The occurrence of multidrug-resistant CCREC capable of transferring their antibiotic resistance genotypes via conjugative plasmids is alarming. WWTPs bring bacteria from different sources together, providing opportunities for horizontal ex-change of DNA among compatible hosts. Further dissemination of the colistin-, carbapenem-, or both colistin-and carbapenem resistant E. coli could lead to a serious threat to public health.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- Escherichia coli
- New Delhi metallo
- ?-lactamase
- mcr-1
- Wastewater treatment plant
- Plasmid
- multidrug resistance
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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