SLC38A10 Deficiency in Mice Affects Plasma Levels of Threonine and Histidine in Males but Not in Females: A Preliminary Characterization Study of SLC38A10(-/-) Mice

Lindberg, Frida A.Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Uppsala Univ, Dept Pharmaceut Biosci, Mol Neuropharmacol, S-75124 Uppsala, Sweden. (author)

SLC38A10 Deficiency in Mice Affects Plasma Levels of Threonine and Histidine in Males but Not in Females : A Preliminary Characterization Study of SLC38A10(-/-) Mice

Solute carriers belong to the biggest group of transporters in the human genome, but more knowledge is needed to fully understand their function and possible role as therapeutic targets. SLC38A10, a poorly characterized solute carrier, is preliminary characterized here. By using a knockout mouse model, we studied the biological effects of SLC38A10 deficiency in vivo. We performed a transcriptomic analysis of the whole brain and found seven differentially expressed genes in SLC38A10-deficient mice (Gm48159, Nr4a1, Tuba1c, Lrrc56, mt-Tp, Hbb-bt and Snord116/9). By measuring amino acids in plasma, we found lower levels of threonine and histidine in knockout males, whereas no amino acid levels were affected in females, suggesting that SLC38A10(-/-) might affect sexes differently. Using RT-qPCR, we investigated the effect of SLC38A10 deficiency on mRNA expression of other SLC38 members, Mtor and Rps6kb1 in the brain, liver, lung, muscle, and kidney, but no differences were found. Relative telomere length measurement was also taken, as a marker for cellular age, but no differences were found between the genotypes. We conclude that SLC38A10 might be important for keeping amino acid homeostasis in plasma, at least in males, but no major effects were seen on transcriptomic expression or telomere length in the whole brain.

Nordenankar, Karin,1981-Uppsala Univ, Dept Pharmaceut Biosci, Mol Neuropharmacol, S-75124 Uppsala, Sweden.(Swepub:uu)karno631 (author)
Forsberg, EricaUppsala universitet,Institutionen för farmaceutisk biovetenskap,Uppsala Univ, Dept Pharmaceut Biosci, Mol Neuropharmacol, S-75124 Uppsala, Sweden. (author)
Fredriksson, RobertUppsala universitet,Institutionen för farmaceutisk biovetenskap,Uppsala Univ, Dept Pharmaceut Biosci, Mol Neuropharmacol, S-75124 Uppsala, Sweden.(Swepub:uu)rfr20930 (author)
Uppsala universitetInstitutionen för farmaceutisk biovetenskap (creator_code:org_t)

By the author/editor: Lindberg, Frida ...; Nordenankar, Kar ...; Forsberg, Erica; Fredriksson, Rob ...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Other Basic Medi ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Physiology

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Neurosciences

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