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SLC38A10 Deficiency...
SLC38A10 Deficiency in Mice Affects Plasma Levels of Threonine and Histidine in Males but Not in Females : A Preliminary Characterization Study of SLC38A10(-/-) Mice
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- Lindberg, Frida A. (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Uppsala Univ, Dept Pharmaceut Biosci, Mol Neuropharmacol, S-75124 Uppsala, Sweden.
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- Nordenankar, Karin, 1981- (författare)
- Uppsala Univ, Dept Pharmaceut Biosci, Mol Neuropharmacol, S-75124 Uppsala, Sweden.
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- Forsberg, Erica (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Uppsala Univ, Dept Pharmaceut Biosci, Mol Neuropharmacol, S-75124 Uppsala, Sweden.
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- Fredriksson, Robert (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Uppsala Univ, Dept Pharmaceut Biosci, Mol Neuropharmacol, S-75124 Uppsala, Sweden.
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(creator_code:org_t)
- 2023-03-30
- 2023
- Engelska.
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Ingår i: Genes. - : MDPI. - 2073-4425. ; 14:4
- Relaterad länk:
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Solute carriers belong to the biggest group of transporters in the human genome, but more knowledge is needed to fully understand their function and possible role as therapeutic targets. SLC38A10, a poorly characterized solute carrier, is preliminary characterized here. By using a knockout mouse model, we studied the biological effects of SLC38A10 deficiency in vivo. We performed a transcriptomic analysis of the whole brain and found seven differentially expressed genes in SLC38A10-deficient mice (Gm48159, Nr4a1, Tuba1c, Lrrc56, mt-Tp, Hbb-bt and Snord116/9). By measuring amino acids in plasma, we found lower levels of threonine and histidine in knockout males, whereas no amino acid levels were affected in females, suggesting that SLC38A10(-/-) might affect sexes differently. Using RT-qPCR, we investigated the effect of SLC38A10 deficiency on mRNA expression of other SLC38 members, Mtor and Rps6kb1 in the brain, liver, lung, muscle, and kidney, but no differences were found. Relative telomere length measurement was also taken, as a marker for cellular age, but no differences were found between the genotypes. We conclude that SLC38A10 might be important for keeping amino acid homeostasis in plasma, at least in males, but no major effects were seen on transcriptomic expression or telomere length in the whole brain.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- SNAT10
- knockout
- amino acid homeostasis
- gene expression analysis
- solute carrier
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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