Coupling microchip pressure regulators with chipHPLC as a step toward fully portable analysis system

• Herein the coupling of a miniaturized, nanoliter scaled, pressure regulator (chipPR), and a chipHPLC device is introduced. The active temperature based flow control of the chipPR is able to generate rapid pressure changes and therefore enables on-chip pinched injection and flow gradients with reduced instrumental effort and minimal dead volumes. The functionality of the chipPR empowered chipHLPC device was demonstrated with high-speed HPLC-separations applying fluorescence and electrospray mass spectrometry (ESI-MS) detection. The system shows excellent long-term stability of chromatography integrity (retention times with RSD of 0.44-0.91%) due to the integration of a PID feedback regulation. This first chip-based HPLC device equipped with chipPRs enables precise flow control with significantly reduced technical effort compared to the state-of-the-art.

Ämnesord

TEKNIK OCH TEKNOLOGIER  -- Elektroteknik och elektronik -- Annan elektroteknik och elektronik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Electrical Engineering, Electronic Engineering, Information Engineering -- Other Electrical Engineering, Electronic Engineering, Information Engineering (hsv//eng)

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

Nyckelord

Lab-on-a-chip

ChipHPLC

Thermal actuation

Microfluidics

Pressure regulation

Publikations- och innehållstyp

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