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Single-cell transcr...
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Li, JiehanStanford Univ, Dept Med, Div Cardiovasc Med, Sch Med, Stanford, CA 94305 USA.;Stanford Univ, Stanford Diabet Res Ctr, Sch Med, Stanford, CA 94305 USA.;Stanford Univ, Stanford Cardiovasc Inst, Sch Med, Stanford, CA 94305 USA.
(författare)
Single-cell transcriptome dataset of human and mouse in vitro adipogenesis models
- Artikel/kapitelEngelska2023
Förlag, utgivningsår, omfång ...
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Springer Nature,2023
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-507494
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-507494URI
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https://doi.org/10.1038/s41597-023-02293-xDOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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Adipogenesis is a process in which fat-specific progenitor cells (preadipocytes) differentiate into adipocytes that carry out the key metabolic functions of the adipose tissue, including glucose uptake, energy storage, and adipokine secretion. Several cell lines are routinely used to study the molecular regulation of adipogenesis, in particular the immortalized mouse 3T3-L1 line and the primary human Simpson-Golabi-Behmel syndrome (SGBS) line. However, the cell-to-cell variability of transcriptional changes prior to and during adipogenesis in these models is not well understood. Here, we present a single-cell RNA-Sequencing (scRNA-Seq) dataset collected before and during adipogenic differentiation of 3T3-L1 and SGBS cells. To minimize the effects of experimental variation, we mixed 3T3-L1 and SGBS cells and used computational analysis to demultiplex transcriptomes of mouse and human cells. In both models, adipogenesis results in the appearance of three cell clusters, corresponding to preadipocytes, early and mature adipocytes. These data provide a groundwork for comparative studies on these widely used in vitro models of human and mouse adipogenesis, and on cell-to-cell variability during this process.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Jin, ChristopherStanford Univ, Dept Med, Div Cardiovasc Med, Sch Med, Stanford, CA 94305 USA.
(författare)
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Gustafsson, StefanUppsala universitet,Klinisk epidemiologi(Swepub:uu)stegu732
(författare)
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Rao, AbhiramStanford Univ, Dept Bioengn, Stanford, CA 94305 USA.
(författare)
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Wabitsch, MartinUlm Univ, Ctr Rare Endocrine Dis, Dept Pediat & Adolescent Med, Div Pediat Endocrinol & Diabet,Med Ctr, D-89075 Ulm, Germany.
(författare)
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Park, Chong Y.Stanford Univ, Dept Med, Div Cardiovasc Med, Sch Med, Stanford, CA 94305 USA.;Stanford Univ, Stanford Diabet Res Ctr, Sch Med, Stanford, CA 94305 USA.;Stanford Univ, Stanford Cardiovasc Inst, Sch Med, Stanford, CA 94305 USA.
(författare)
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Quertermous, ThomasStanford Univ, Dept Med, Div Cardiovasc Med, Sch Med, Stanford, CA 94305 USA.;Stanford Univ, Stanford Cardiovasc Inst, Sch Med, Stanford, CA 94305 USA.
(författare)
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Knowles, Joshua W.Stanford Univ, Dept Med, Div Cardiovasc Med, Sch Med, Stanford, CA 94305 USA.;Stanford Univ, Stanford Diabet Res Ctr, Sch Med, Stanford, CA 94305 USA.;Stanford Univ, Stanford Cardiovasc Inst, Sch Med, Stanford, CA 94305 USA.;Stanford Univ, Stanford Prevent Res Ctr, Sch Med, Stanford, CA 94305 USA.
(författare)
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Bielczyk-Maczynska, EwaStanford Univ, Dept Med, Div Cardiovasc Med, Sch Med, Stanford, CA 94305 USA.;Stanford Univ, Stanford Diabet Res Ctr, Sch Med, Stanford, CA 94305 USA.;Stanford Univ, Stanford Cardiovasc Inst, Sch Med, Stanford, CA 94305 USA.
(författare)
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Stanford Univ, Dept Med, Div Cardiovasc Med, Sch Med, Stanford, CA 94305 USA.;Stanford Univ, Stanford Diabet Res Ctr, Sch Med, Stanford, CA 94305 USA.;Stanford Univ, Stanford Cardiovasc Inst, Sch Med, Stanford, CA 94305 USA.Stanford Univ, Dept Med, Div Cardiovasc Med, Sch Med, Stanford, CA 94305 USA.
(creator_code:org_t)
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Ingår i:Scientific Data: Springer Nature102052-4463
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