Sökning: id:"swepub:oai:DiVA.org:uu-508120" >
Is islet amyloid po...
-
Libard, SylwiaUppsala universitet,Institutionen för immunologi, genetik och patologi,Uppsala Univ Hosp, Dept Surg Pathol, S-75185 Uppsala, Sweden
(författare)
Is islet amyloid polypeptide indeed expressed in the human brain?
- Artikel/kapitelEngelska2023
Förlag, utgivningsår, omfång ...
-
John Wiley & Sons,2023
-
electronicrdacarrier
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:uu-508120
-
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-508120URI
-
https://doi.org/10.1111/nan.12917DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
Aims This study aims to study the association between pancreatic islet amyloid polypeptide (IAPP) and Alzheimer's disease neuropathological change (ADNC) in brain biopsies obtained from subjects with idiopathic normal pressure hydrocephalus (iNPH) and in post-mortem (PM) brain samples obtained from aged individuals.Methods For the immunohistochemical (IHC) analyses, two IAPP antibodies (Abs), monoclonal and polyclonal, and Abs directed towards ADNC were applied.Results The iNPH cohort included 113 subjects. Amyloid-& beta; (A & beta;) was detected in 50% and hyperphosphorylated & tau; (HP & tau;) in 47% of the cases. Concomitant pathology was seen in 32%. The PM cohort included 77 subjects. A & beta; was detected in 69% and HP & tau; in 91% of the cases. Combined A & beta;/HP & tau; pathology was seen in 62%. Reactivity for the monoclonal IAPP was not detected in the brain tissue in either of the cohorts. Reactivity for the polyclonal IAPP was observed in all 77 PM brain samples.Conclusions There was no specific expression of IAPP in human brain tissue; hence, an association between IAPP and ADNC is not assessable. Of note, the observed reactivity of the polyclonal IAPP Ab was not reproduced with a specific monoclonal Ab; thus, we considered the observed staining with the polyclonal Ab to be unreliable. When using IHC, several pitfalls, especially the choice of an Ab, always need to be considered. Polyclonal Abs cross-react with other epitopes and proteins, thus leading to false-positive results. This seems to be the case for the polyclonal IAPP Abs in the human brain.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Alafuzoff, IrinaUppsala Univ Hosp, Dept Pathol, Uppsala, Sweden
(författare)
-
Uppsala universitetInstitutionen för immunologi, genetik och patologi
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Neuropathology and Applied Neurobiology: John Wiley & Sons49:40305-18461365-2990
Internetlänk
Hitta via bibliotek
Till lärosätets databas