Correlative image analysis for in-depth chemical analysis using multiple parallelized mass spectrometry imaging with high resolution and MSn

• Attaining specificity in mass spectrometry imaging (MSI) is challenging and often results in longer experimental runtime or lower spatial resolution. Herein we describe a novel method for acquisition of MS2 spectra in an ion trap parallel with high resolution accurate mass FTMS scans. Using the nanospray desorption electrospray ionization (nano-DESI) ion source for MSI, we were able to perform 28 MS2 events in an ion trap simultaneously to recording a transient for FTMS. This allowed for deep characterization of the targeted compounds and allowed for improved annotation by spatially correlating the FTMS and ITMS2 data. By combining the data streams from data with different specificities we were able to efficiently data mine the complex data set and could annotate several lipid species. 

Ämnesord

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

Nyckelord

Mass spectrometry imaging

parallel

parallel acquisition

MS/MS

MSn

lipid

phospholipid

Kemi med inriktning mot analytisk kemi

Chemistry with specialization in Analytical Chemistry

Publikations- och innehållstyp

vet (ämneskategori)

ovr (ämneskategori)