Two granzyme A/K homologs in Zebra mbuna have different specificities, one classical tryptase and one with chymase activity

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Sökning: L773:1879 0089 > Two granzyme A/K ho...

Aybay, ErdemUppsala universitet,Institutionen för cell- och molekylärbiologi (författare)

Two granzyme A/K homologs in Zebra mbuna have different specificities, one classical tryptase and one with chymase activity

Artikel/kapitelEngelska2023

Förlag, utgivningsår, omfång ...

Elsevier,2023
electronicrdacarrier

Nummerbeteckningar

LIBRIS-ID:oai:DiVA.org:uu-514069
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-514069URI
https://doi.org/10.1016/j.dci.2023.104920DOI
https://res.slu.se/id/publ/126351URI

Kompletterande språkuppgifter

Språk:engelska
Sammanfattning på:engelska

Ingår i deldatabas

SwePubSwePub

Klassifikation

Ämneskategori:ref swepub-contenttype
Ämneskategori:art swepub-publicationtype

Anmärkningar

Granzymes A and K are two highly homologous serine proteases expressed by mammalian cytotoxic T cells (CTLs) and natural killer (NK) cells. The locus encoding these two proteases is the first of the hematopoietic serine protease loci to appear during vertebrate evolution. This locus is found in all jawed vertebrates including the cartilaginous fishes. Granzyme A is the most abundant of the different granzymes expressed by CTLs and NK cells and its potential function has been studied extensively for many years. However, no clear conclusions concerning its primary role in the immune defense has been obtained. In all mammals, there are only one copy each of granzyme A and K, whereas additional copies are found in both cartilaginous and ray finned fishes. In cichlids two of these copies seem to encode new members of the granzyme A/K family. These two new members appear to have changed primary specificity and to be pure chymases based on the amino acids in their active site substrate binding pockets. Interestingly, one of these gene copies is located in the middle of the granzyme A/K locus, while the other copy is present in another locus, the met-ase locus. We here present a detailed characterization of the extended cleavage specificity of one of these non-classical granzymes, a Zebra mbuna granzyme positioned in the granzyme A/K locus. This enzyme, named granzyme A2, showed a high preference for tyrosine in the P1 position of substrates, thereby being a strict chymase. We have also characterized one of the classical granzyme A/Ks of the Zebra mbuna, granzyme A1, which is a tryptase with preference for arginine in the P1 position of substrates. Based on their extended specificities, the two granzymes showed major similarities, but also some differences in preferred amino acids in positions surrounding the cleavable amino acid. Fish lack one of the hematopoietic serine protease loci of mammals, the chymase locus, where one of the major mast cell enzymes is located. An interesting question is now if cichlids have by compensatory mechanisms generated a mast cell chymase from another locus, and if similar chymotryptic enzymes have appeared also in other fish species.

Ämnesord och genrebeteckningar

NATURVETENSKAP Biologi Immunologi hsv//swe
NATURAL SCIENCES Biological Sciences Immunology hsv//eng
NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
LANTBRUKSVETENSKAPER Lantbruksvetenskap, skogsbruk och fiske Fisk- och akvakulturforskning hsv//swe
AGRICULTURAL SCIENCES Agriculture, Forestry and Fisheries Fish and Aquacultural Science hsv//eng
Cytotoxic T cells
NK cell
Granzyme A
Apoptosis
Caspase
Chymase

Biuppslag (personer, institutioner, konferenser, titlar ...)

Elkhalifa, MamounUppsala universitet,Institutionen för cell- och molekylärbiologi (författare)
Akula, SrinivasSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Mikrobiologi och immunologi,Swedish Univ Agr Sci, Dept Anat Physiol & Biochem, Box 7011, SE-75007 Uppsala, Sweden.,Institutionen för anatomi, fysiologi och biokemi (AFB),Department of Anatomy, Physiology and Biochemistry (AFB),Uppsala University(Swepub:uu)akusr139 (författare)
Wernersson, SaraSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för anatomi, fysiologi och biokemi (AFB),Department of Anatomy, Physiology and Biochemistry (AFB),Swedish Univ Agr Sci, Dept Anat Physiol & Biochem, Box 7011, SE-75007 Uppsala, Sweden.(Swepub:slu)48577 (författare)
Hellman, LarsUppsala universitet,Mikrobiologi och immunologi(Swepub:uu)larshell (författare)
Uppsala universitetInstitutionen för cell- och molekylärbiologi (creator_code:org_t)
Sveriges lantbruksuniversitet

Sammanhörande titlar

Ingår i:Developmental and Comparative Immunology: Elsevier1480145-305X1879-0089

Internetlänk

Hitta via bibliotek

Developmental and Comparative Immunology (Sök värdpublikationen i LIBRIS)

Till lärosätets databas

Hitta mer i SwePub

Av författaren/redakt...: Aybay, Erdem; Elkhalifa, Mamou ...; Akula, Srinivas; Wernersson, Sara; Hellman, Lars

Om ämnet

NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Immunologi

NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Biokemi och mole ...

LANTBRUKSVETENSKAPER: LANTBRUKSVETENSK ...; och Lantbruksvetensk ...; och Fisk och akvakul ...

Artiklar i publikationen: Developmental an ...

Av lärosätet: Uppsala universitet; Sveriges Lantbruksuniversitet

Sök utanför SwePub

Sök vidare i:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se