id:"swepub:oai:DiVA.org:uu-515564"
Search: id:"swepub:oai:DiVA.org:uu-515564" > Insights into the m...
- 1 of 1
- Previous record
- Next record
- To hitlist
- Wang, Hao-Tian (author)
Insights into the missing apiosylation step in flavonoid apiosides biosynthesis of Leguminosae plants
- Article/chapterEnglish2023
Publisher, publication year, extent ...
- Springer Nature,2023
- electronicrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:uu-515564
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-515564URI
- https://doi.org/10.1038/s41467-023-42393-1DOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- Apiose is a natural pentose containing an unusual branched-chain structure. Apiosides are bioactive natural products widely present in the plant kingdom. However, little is known on the key apiosylation reaction in the biosynthetic pathways of apiosides. In this work, we discover an apiosyltransferase GuApiGT from Glycyrrhiza uralensis. GuApiGT could efficiently catalyze 2″-O-apiosylation of flavonoid glycosides, and exhibits strict selectivity towards UDP-apiose. We further solve the crystal structure of GuApiGT, determine a key sugar-binding motif (RLGSDH) through structural analysis and theoretical calculations, and obtain mutants with altered sugar selectivity through protein engineering. Moreover, we discover 121 candidate apiosyltransferase genes from Leguminosae plants, and identify the functions of 4 enzymes. Finally, we introduce GuApiGT and its upstream genes into Nicotiana benthamiana, and complete de novo biosynthesis of a series of flavonoid apiosides. This work reports an efficient phenolic apiosyltransferase, and reveals mechanisms for its sugar donor selectivity.
Subject headings and genre
- NATURVETENSKAP Biologi Strukturbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Structural Biology hsv//eng
- NATURVETENSKAP Kemi Teoretisk kemi hsv//swe
- NATURAL SCIENCES Chemical Sciences Theoretical Chemistry hsv//eng
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
Added entries (persons, corporate bodies, meetings, titles ...)
- Wang, Zi-Long (author)
- Chen, Kuan (author)
- Yao, Ming-Ju (author)
- Zhang, Meng (author)
- Wang, Rong-Shen (author)
- Zhang, Jia-He (author)
- Ågren, HansUppsala universitet,Kemisk och biomolekylär fysik(Swepub:uu)hanag788 (author)
- Li, Fu-Dong (author)
- Li, JunhaoUppsala universitet,Kemisk och biomolekylär fysik(Swepub:uu)jonli333 (author)
- Qiao, Xue (author)
- Ye, Min (author)
- Uppsala universitetKemisk och biomolekylär fysik (creator_code:org_t)
Related titles
- In:Nature Communications: Springer Nature14:12041-1723
Internet link
Find in a library
- Nature Communications (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Wang, Hao-Tian
- Wang, Zi-Long
- Chen, Kuan
- Yao, Ming-Ju
- Zhang, Meng
- Wang, Rong-Shen
- show more...
- Zhang, Jia-He
- Ågren, Hans
- Li, Fu-Dong
- Li, Junhao
- Qiao, Xue
- Ye, Min
- show less...
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- and Structural Biolo ...
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Chemical Science ...
- and Theoretical Chem ...
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- and Biochemistry and ...
- Articles in the publication
- Nature Communica ...
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
