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Microfluidic-based ...
Microfluidic-based production of [68Ga]Ga-FAPI-46 and [68Ga]Ga-DOTA-TOC using the cassette-based iMiDEV⢠microfluidic radiosynthesizer
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- Mallapura, Hemantha (författare)
- Karolinska Inst, Ctr Psychiat Res, Dept Clin Neurosci, SE-17176 Stockholm, Sweden.;Stockholm Cty Council, SE-17176 Stockholm, Sweden.
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- Ovdiichuk, Olga (författare)
- Nancyclotep, Mol Imaging Platform, 5 Rue Morvan, F-54500 Vandoeuvre Les Nancy, France.
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- Jussing, Emma (författare)
- Karolinska Inst, Dept Oncol & Pathol, S-17177 Stockholm, Sweden.;Karolinska Univ Hosp, Dept Radiopharm, S-17176 Stockholm, Sweden.
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- Thuy, Tran A. (författare)
- Karolinska Inst, Dept Oncol & Pathol, S-17177 Stockholm, Sweden.;Karolinska Univ Hosp, Dept Radiopharm, S-17176 Stockholm, Sweden.
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- Piatkowski, Camille (författare)
- PMB Alcen, Route Michels CD56, F-13790 Peynier, France.
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- Tanguy, Laurent (författare)
- PMB Alcen, Route Michels CD56, F-13790 Peynier, France.
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- Collet-Defossez, Charlotte (författare)
- Nancyclotep, Mol Imaging Platform, 5 Rue Morvan, F-54500 Vandoeuvre Les Nancy, France.;Univ Lorraine, Inserm, IADI, F-54000 Nancy, France.
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- Långström, Bengt (författare)
- Uppsala universitet,Institutionen för kemi - BMC
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- Halldin, Christer (författare)
- Karolinska Inst, Ctr Psychiat Res, Dept Clin Neurosci, SE-17176 Stockholm, Sweden.;Stockholm Cty Council, SE-17176 Stockholm, Sweden.
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- Nag, Sangram (författare)
- Karolinska Institutet
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Karolinska Inst, Ctr Psychiat Res, Dept Clin Neurosci, SE-17176 Stockholm, Sweden;Stockholm Cty Council, SE-17176 Stockholm, Sweden. Nancyclotep, Mol Imaging Platform, 5 Rue Morvan, F-54500 Vandoeuvre Les Nancy, France. (creator_code:org_t)
- Springer, 2023
- 2023
- Engelska.
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Ingår i: EJNMMI Radiopharmacy and Chemistry. - : Springer. - 2365-421X. ; 8:1
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Background The demand for Ga-68-labeled radiotracers has significantly increased in the past decade, driven by the development of diversified imaging tracers, such as FAPI derivatives, PSMA-11, DOTA-TOC, and DOTA-TATE. These tracers have exhibited promising results in theranostic applications, fueling interest in exploring them for clinical use. Among these probes, Ga-68-labeled FAPI-46 and DOTA-TOC have emerged as key players due to their ability to diagnose a broad spectrum of cancers ([Ga-68]Ga-FAPI-46) in late-phase studies, whereas [Ga-68]Ga-DOTA-TOC is clinically approved for neuroendocrine tumors. To facilitate their production, we leveraged a microfluidic cassette-based iMiDEV radiosynthesizer, enabling the synthesis of [Ga-68]Ga-FAPI-46 and [Ga-68]Ga-DOTA-TOC based on a dose-on-demand (DOD) approach.Results Different mixing techniques were explored to influence radiochemical yield. We achieved decay-corrected yield of 44 +/- 5% for [Ga-68]Ga-FAPI-46 and 46 +/- 7% for [Ga-68]Ga-DOTA-TOC in approximately 30 min. The radiochemical purities (HPLC) of [Ga-68]Ga-FAPI-46 and [Ga-68]Ga-DOTA-TOC were 98.2 +/- 0.2% and 98.4 +/- 0.9%, respectively. All the quality control results complied with European Pharmacopoeia quality standards. We optimized various parameters, including Ga-68 trapping and elution, cassette batches, passive mixing in the reactor, and solid-phase extraction (SPE) purification and formulation. The developed synthesis method reduced the amount of precursor and other chemicals required for synthesis compared to conventional radiosynthesizers.Conclusions The microfluidic-based approach enabled the implementation of radiosynthesis of [Ga-68]Ga-FAPI-46 and [Ga-68]Ga-DOTA-TOC on the iMiDEV (TM) microfluidic module, paving the way for their use in preclinical and clinical applications. The microfluidic synthesis approach utilized 2-3 times less precursor than cassette-based conventional synthesis. The synthesis method was also successfully validated in a similar microfluidic iMiDEV module at a different research center for the synthesis of [Ga-68]Ga-FAPI-46 with limited runs. Our study demonstrated the potential of microfluidic methods for efficient and reliable radiometal-based radiopharmaceutical synthesis, contributing valuable insights for future advancements in this field and paving the way for routine clinical applications in the near future.
Ämnesord
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Nyckelord
- Positron emission tomography (PET)
- Radiopharmaceuticals
- Microfluidics
- iMiDEV
- [Ga-68]Ga-FAPI-46
- [Ga-68]Ga-DOTA-TOC
- Radiolabeling
- Dose-on-demand (DOD)
- Radiotracers
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Mallapura, Heman ...
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Ovdiichuk, Olga
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Jussing, Emma
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Thuy, Tran A.
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Piatkowski, Cami ...
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Tanguy, Laurent
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Collet-Defossez, ...
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Långström, Bengt
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Halldin, Christe ...
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Nag, Sangram
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Organisk kemi
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EJNMMI Radiophar ...
- Av lärosätet
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Uppsala universitet
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Karolinska Institutet