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Equine enteroid-der...
Equine enteroid-derived monolayers recapitulate key features of parasitic intestinal nematode infection
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- Hellman, Stina (författare)
- Swedish Univ Agr Sci, Dept Biomed Sci & Vet Publ Hlth, SLU, POB 7028, S-75007 Uppsala, Sweden.
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- Martin, Frida (författare)
- Swedish Univ Agr Sci, Dept Biomed Sci & Vet Publ Hlth, SLU, POB 7028, S-75007 Uppsala, Sweden.
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- Tyden, Eva (författare)
- Swedish Univ Agr Sci, Dept Biomed Sci & Vet Publ Hlth, SLU, POB 7028, S-75007 Uppsala, Sweden.
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- Sellin, Mikael E. (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk biokemi och mikrobiologi
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- Norman, Albin (författare)
- Swedish Univ Agr Sci, Dept Biomed Sci & Vet Publ Hlth, SLU, POB 7028, S-75007 Uppsala, Sweden.
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- Hjertner, Bernt (författare)
- Swedish Univ Agr Sci, Dept Biomed Sci & Vet Publ Hlth, SLU, POB 7028, S-75007 Uppsala, Sweden.
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- Svedberg, Pia (författare)
- Vidilab AB, POB 33, S-74521 Enkoping, Sweden.
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- Fossum, Caroline (författare)
- Swedish Univ Agr Sci, Dept Biomed Sci & Vet Publ Hlth, SLU, POB 7028, S-75007 Uppsala, Sweden.
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Swedish Univ Agr Sci, Dept Biomed Sci & Vet Publ Hlth, SLU, POB 7028, S-75007 Uppsala, Sweden Science for Life Laboratory, SciLifeLab (creator_code:org_t)
- Springer Nature, 2024
- 2024
- Engelska.
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Ingår i: Veterinary research (Print). - : Springer Nature. - 0928-4249 .- 1297-9716. ; 55:1
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Stem cell-derived organoid cultures have emerged as attractive experimental models for infection biology research regarding various types of gastro-intestinal pathogens and host species. However, the large size of infectious nematode larvae and the closed structure of 3-dimensional organoids often hinder studies of the natural route of infection. To enable easy administration to the apical surface of the epithelium, organoids from the equine small intestine, i.e. enteroids, were used in the present study to establish epithelial monolayer cultures. These monolayers were functionally tested by stimulation with IL-4 and IL-13, and/or exposure to infectious stage larvae of the equine nematodes Parascaris univalens, cyathostominae and/or Strongylus vulgaris. Effects were recorded using transcriptional analysis combined with histochemistry, immunofluorescence-, live-cell- and scanning electron microscopy. These analyses revealed heterogeneous monolayers containing both immature and differentiated cells including tuft cells and mucus-producing goblet cells. Stimulation with IL-4/IL-13 increased tuft- and goblet cell differentiation as demonstrated by the expression of DCLK1 and MUC2. In these cytokine-primed monolayers, the expression of MUC2 was further promoted by co-culture with P. univalens. Moreover, live-cell imaging revealed morphological alterations of the epithelial cells following exposure to larvae even in the absence of cytokine stimulation. Thus, the present work describes the design, characterization and usability of an experimental model representing the equine nematode-infected small intestinal epithelium. The presence of tuft cells and goblet cells whose mucus production is affected by Th2 cytokines and/or the presence of larvae opens up for mechanistic studies of the physical interactions between nematodes and the equine intestinal mucosa.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- LANTBRUKSVETENSKAPER -- Veterinärmedicin -- Klinisk vetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Veterinary Science -- Clinical Science (hsv//eng)
- LANTBRUKSVETENSKAPER -- Veterinärmedicin -- Patobiologi (hsv//swe)
- AGRICULTURAL SCIENCES -- Veterinary Science -- Pathobiology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Nyckelord
- Equine
- organoid
- enteroid
- enteroid-derived
- monolayer
- nematode
- larvae
- intestine
- in vitro
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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