Characterization of a Novel Sulfated Carbohydrate Unit Implicated in the Carbohydrate­Carbohydrate-mediated Cell Aggregation of the Marine Sponge Microciona prolifera

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Spillmann, DorotheUniversity of Turku (author)

Characterization of a Novel Sulfated Carbohydrate Unit Implicated in the CarbohydrateCarbohydrate-mediated Cell Aggregation of the Marine Sponge Microciona prolifera

Article/chapterEnglish1995

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1995
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LIBRIS-ID:oai:DiVA.org:uu-52622
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-52622URI

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Language:English
Summary in:English

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SwePubSwePub

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Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Species-specific cell reaggregation in the marine sponge Microciona prolifera is mediated by an adhesion proteoglycan. Two interactions are involved in the process: a Ca(2+)-dependent homophilic binding between proteoglycan molecules and a Ca(2+)-independent binding between the molecule and cells. Both interactions are mediated by the glycan moieties of the proteoglycan. The interaction of the proteoglycan with itself has been characterized as a carbohydrate-carbohydrate interaction of multiple low affinity sites. The monoclonal antibodies Block 1 and Block 2 raised against the purified aggregation proteoglycan and selected for inhibition of aggregation bind to these glycans. In a previous report the structure, [formula: see text] was assigned to the oligosaccharide reacting with Block 1 antibody (Spillmann, D., Hård, K., Thomas-Oates, J., Vliegenthart, J. F. G., Misevic, G., Burger, M. M., and Finne, J. (1993) J. Biol. Chem. 268, 13378-13387). By the technique of attaching the water-soluble acid-degraded fragments to a lipid carrier for immunochemical detection and by chemical, enzymatic and spectroscopic methods the structure, [formula: see text] was assigned to the oligosaccharide reacting with the aggregation-blocking monoclonal antibody Block 2. The structure, [formula: see text] was assigned to a major nonreactive oligosaccharide, which outlined the molecular requirements of antibody binding of the two aggregation-associated epitopes. These data demonstrate that two different functional sites with distinct structural characteristics and antibody reactivities are involved in the reaggregation of sponge cells, a model of carbohydrate-carbohydrate-mediated cell interactions.

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MEDICINE
MEDICIN

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Thomas-Oates, J.E. (author)
van Kuik, A (author)
Vliegenhart, J.F.G. (author)
Misevic, G (author)
Burger, M.M. (author)
Finne, J (author)
University of Turku (creator_code:org_t)

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In:Journal of Biological Chemistry270, s. 5089-50970021-92581083-351X

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