ProQ-dependent activation of Salmonella virulence genes mediated by post-transcriptional control of PhoP synthesis

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Berggren, SofiaUppsala universitet,Mikrobiologi och immunologi (author)

ProQ-dependent activation of Salmonella virulence genes mediated by post-transcriptional control of PhoP synthesis

Article/chapterEnglish2024

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American Society for Microbiology,2024
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LIBRIS-ID:oai:DiVA.org:uu-528156
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-528156URI
https://doi.org/10.1128/msphere.00018-24DOI

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Language:English
Summary in:English

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Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Gastrointestinal disease caused by Salmonella enterica is associated with the pathogen's ability to replicate within epithelial cells and macrophages. Upon host cell entry, the bacteria express a type-three secretion system encoded within Salmonella pathogenicity island 2, through which host-manipulating effector proteins are secreted to establish a stable intracellular niche. Transcription of this intracellular virulence program is activated by the PhoPQ two-component system that senses the low pH and the reduced magnesium concentration of host cell vacuoles. In addition to transcriptional control, Salmonella commonly employ RNA-binding proteins (RBPs) and small regulatory RNAs (sRNAs) to regulate gene expression at the post-transcriptional level. ProQ is a globally acting RBP in Salmonella that promotes expression of the intracellular virulence program, but its RNA repertoire has previously been characterized only under standard laboratory growth conditions. Here, we provide a high-resolution ProQ interactome during conditions mimicking the environment of the Salmonella-containing vacuole (SCV), revealing hundreds of previously unknown ProQ binding sites in sRNAs and mRNA 3 ' UTRs. ProQ positively affected both the levels and the stability of many sRNA ligands, some of which were previously shown to associate with the well-studied and infection-relevant RBP Hfq. We further show that ProQ activates the expression of PhoP at the post-transcriptional level, which, in turn, leads to upregulation of the intracellular virulence program.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Mikrobiologi inom det medicinska området hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Microbiology in the medical area hsv//eng
NATURVETENSKAP Biologi Mikrobiologi hsv//swe
NATURAL SCIENCES Biological Sciences Microbiology hsv//eng
ProQ
CLIP-seq
RNA-binding protein
small RNA
Salmonella
virulence
PhoP

Added entries (persons, corporate bodies, meetings, titles ...)

Andresen, LiisUppsala universitet,Institutionen för cell- och molekylärbiologi(Swepub:uu)liian191 (author)
Kjellin, JonasUppsala universitet,Mikrobiologi och immunologi(Swepub:uu)jonkj713 (author)
Burgo, Yolanda MartinezUppsala universitet,Institutionen för cell- och molekylärbiologi (author)
Geiser, PetraUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)petma163 (author)
Baars, SophieUppsala universitet,Institutionen för cell- och molekylärbiologi (author)
Söderbom, FredrikUppsala universitet,Mikrobiologi och immunologi(Swepub:uu)fredsbom (author)
Sellin, Mikael E.Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk biokemi och mikrobiologi(Swepub:uu)mikse125 (author)
Holmqvist, Erik,1977-Uppsala universitet,Mikrobiologi och immunologi(Swepub:uu)erhol103 (author)
Uppsala universitetMikrobiologi och immunologi (creator_code:org_t)

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In:mSphere: American Society for Microbiology9:32379-5042

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By the author/editor: Berggren, Sofia; Andresen, Liis; Kjellin, Jonas; Burgo, Yolanda M ...; Geiser, Petra; Baars, Sophie; show more...; Söderbom, Fredri ...; Sellin, Mikael E ...; Holmqvist, Erik, ...; show less...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Microbiology in ...

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