Search: WFRF:(Baars E) >
ProQ-dependent acti...
ProQ-dependent activation of Salmonella virulence genes mediated by post-transcriptional control of PhoP synthesis
-
- Berggren, Sofia (author)
- Uppsala universitet,Mikrobiologi och immunologi
-
- Andresen, Liis (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
-
- Kjellin, Jonas (author)
- Uppsala universitet,Mikrobiologi och immunologi
-
show more...
-
- Burgo, Yolanda Martinez (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
-
- Geiser, Petra (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Science for Life Laboratory, SciLifeLab
-
- Baars, Sophie (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
-
- Söderbom, Fredrik (author)
- Uppsala universitet,Mikrobiologi och immunologi
-
- Sellin, Mikael E. (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk biokemi och mikrobiologi
-
- Holmqvist, Erik, 1977- (author)
- Uppsala universitet,Mikrobiologi och immunologi
-
show less...
-
(creator_code:org_t)
- American Society for Microbiology, 2024
- 2024
- English.
-
In: mSphere. - : American Society for Microbiology. - 2379-5042. ; 9:3
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://uu.diva-port... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Gastrointestinal disease caused by Salmonella enterica is associated with the pathogen's ability to replicate within epithelial cells and macrophages. Upon host cell entry, the bacteria express a type-three secretion system encoded within Salmonella pathogenicity island 2, through which host-manipulating effector proteins are secreted to establish a stable intracellular niche. Transcription of this intracellular virulence program is activated by the PhoPQ two-component system that senses the low pH and the reduced magnesium concentration of host cell vacuoles. In addition to transcriptional control, Salmonella commonly employ RNA-binding proteins (RBPs) and small regulatory RNAs (sRNAs) to regulate gene expression at the post-transcriptional level. ProQ is a globally acting RBP in Salmonella that promotes expression of the intracellular virulence program, but its RNA repertoire has previously been characterized only under standard laboratory growth conditions. Here, we provide a high-resolution ProQ interactome during conditions mimicking the environment of the Salmonella-containing vacuole (SCV), revealing hundreds of previously unknown ProQ binding sites in sRNAs and mRNA 3 ' UTRs. ProQ positively affected both the levels and the stability of many sRNA ligands, some of which were previously shown to associate with the well-studied and infection-relevant RBP Hfq. We further show that ProQ activates the expression of PhoP at the post-transcriptional level, which, in turn, leads to upregulation of the intracellular virulence program.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- ProQ
- CLIP-seq
- RNA-binding protein
- small RNA
- Salmonella
- virulence
- PhoP
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
-
mSphere
(Search for host publication in LIBRIS)
To the university's database
- By the author/editor
-
Berggren, Sofia
-
Andresen, Liis
-
Kjellin, Jonas
-
Burgo, Yolanda M ...
-
Geiser, Petra
-
Baars, Sophie
-
show more...
-
Söderbom, Fredri ...
-
Sellin, Mikael E ...
-
Holmqvist, Erik, ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Microbiology in ...
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Microbiology
- Articles in the publication
-
mSphere
- By the university
-
Uppsala University