Sökning: WFRF:(Gabriel Christoph)
> (2020-2024) >
Direct Salmonella i...
Direct Salmonella injection into enteroid cells allows the study of host-pathogen interactions in the cytosol with high spatiotemporal resolution
-
- Ernst, Chantal (författare)
- Swiss Fed Inst Technol, Inst Microbiol, Dept Biol, Zurich, Switzerland.
-
- Andreassen, Patrick R. (författare)
- Swiss Fed Inst Technol, Inst Microbiol, Dept Biol, Zurich, Switzerland.
-
- Giger, Gabriel H. (författare)
- Swiss Fed Inst Technol, Inst Microbiol, Dept Biol, Zurich, Switzerland.
-
visa fler...
-
- Nguyen, Bidong D. (författare)
- Swiss Fed Inst Technol, Inst Microbiol, Dept Biol, Zurich, Switzerland.
-
- Gabelein, Christoph G. (författare)
- Swiss Fed Inst Technol, Inst Microbiol, Dept Biol, Zurich, Switzerland.
-
- Guillaume-Gentil, Orane (författare)
- Swiss Fed Inst Technol, Inst Microbiol, Dept Biol, Zurich, Switzerland.
-
- Fattinger, Stefan A. (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Science for Life Laboratory, SciLifeLab,Swiss Fed Inst Technol, Inst Microbiol, Dept Biol, Zurich, Switzerland
-
- Sellin, Mikael E. (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk biokemi och mikrobiologi
-
- Hardt, Wolf-Dietrich (författare)
- Swiss Fed Inst Technol, Inst Microbiol, Dept Biol, Zurich, Switzerland.
-
- Vorholt, Julia A. (författare)
- Swiss Fed Inst Technol, Inst Microbiol, Dept Biol, Zurich, Switzerland.
-
visa färre...
-
Swiss Fed Inst Technol, Inst Microbiol, Dept Biol, Zurich, Switzerland Institutionen för medicinsk biokemi och mikrobiologi (creator_code:org_t)
- Public Library of Science (PLoS), 2024
- 2024
- Engelska.
-
Ingår i: PLoS biology. - : Public Library of Science (PLoS). - 1544-9173 .- 1545-7885. ; 22:4
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://uu.diva-port... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Intestinal epithelial cells (IECs) play pivotal roles in nutrient uptake and in the protection against gut microorganisms. However, certain enteric pathogens, such as Salmonella enterica serovar Typhimurium (S. Tm), can invade IECs by employing flagella and type III secretion systems (T3SSs) with cognate effector proteins and exploit IECs as a replicative niche. Detection of flagella or T3SS proteins by IECs results in rapid host cell responses, i.e., the activation of inflammasomes. Here, we introduce a single-cell manipulation technology based on fluidic force microscopy (FluidFM) that enables direct bacteria delivery into the cytosol of single IECs within a murine enteroid monolayer. This approach allows to specifically study pathogen-host cell interactions in the cytosol uncoupled from preceding events such as docking, initiation of uptake, or vacuole escape. Consistent with current understanding, we show using a live-cell inflammasome reporter that exposure of the IEC cytosol to S. Tm induces NAIP/NLRC4 inflammasomes via its known ligands flagellin and T3SS rod and needle. Injected S. Tm mutants devoid of these invasion-relevant ligands were able to grow in the cytosol of IECs despite the absence of T3SS functions, suggesting that, in the absence of NAIP/NLRC4 inflammasome activation and the ensuing cell death, no effector-mediated host cell manipulation is required to render the epithelial cytosol growth-permissive for S. Tm. Overall, the experimental system to introduce S. Tm into single enteroid cells enables investigations into the molecular basis governing host-pathogen interactions in the cytosol with high spatiotemporal resolution.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas