Sökning: WFRF:(Lanekoff Ingela 1975)
> (2020-2024) >
Interleukin-13 Trea...
Interleukin-13 Treatment of Living Lung Tissue Model Alters the Metabolome and Proteome : A Nano-DESI MS Metabolomics and Shotgun Proteomics Study
-
- Tóth, Gábor (författare)
- Uppsala universitet,Analytisk kemi
-
- Golubova, Anastasia, 1996- (författare)
- Uppsala universitet,Analytisk kemi
-
- Falk, Alexander, Research engineer, 1986- (författare)
- Uppsala universitet,Analytisk kemi
-
visa fler...
-
- Bergström Lind, Sara, 1977- (författare)
- Uppsala universitet,Analytisk kemi
-
- Nicholas, Mark (författare)
- AstraZeneca R&D, S-43183 Mölndal, Sweden.
-
- Lanekoff, Ingela, Prof. 1975- (författare)
- Uppsala universitet,Analytisk kemi
-
visa färre...
-
(creator_code:org_t)
- MDPI, 2024
- 2024
- Engelska.
-
Ingår i: International Journal of Molecular Sciences. - : MDPI. - 1661-6596 .- 1422-0067. ; 25:9
- Relaterad länk:
-
https://doi.org/10.3...
-
visa fler...
-
https://uu.diva-port... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.3...
-
visa färre...
Abstract
Ämnesord
Stäng
- Asthma is a chronic respiratory disease with one of the largest numbers of cases in the world; thus, constant investigation and technical development are needed to unravel the underlying biochemical mechanisms. In this study, we aimed to develop a nano-DESI MS method for the in vivo characterization of the cellular metabolome. Using air-liquid interface (ALI) cell layers, we studied the role of Interleukin-13 (IL-13) on differentiated lung epithelial cells acting as a lung tissue model. We demonstrate the feasibility of nano-DESI MS for the in vivo monitoring of basal-apical molecular transport, and the subsequent endogenous metabolic response, for the first time. Conserving the integrity of the ALI lung-cell layer enabled us to perform temporally resolved metabolomic characterization followed by "bottom-up" proteomics on the same population of cells. Metabolic remodeling was observed upon histamine and corticosteroid treatment of the IL-13-exposed lung cell monolayers, in correlation with alterations in the proteomic profile. This proof of principle study demonstrates the utility of in vivo nano-DESI MS for characterizing ALI tissue layers, and the new markers identified in our study provide a good starting point for future, larger-scale studies.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- asthma
- interleukin-13
- nano-DESI
- proteomics
- metabolomics
- multi-omics
- air-liquid interface
- amino acid
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas