One-Tip enables comprehensive proteome coverage in minimal cells and single zygotes

Ye, ZiluChinese Acad Med Sci & Peking Union Med Coll, State Key Lab Common Mech Res Major Dis, Suzhou Inst Syst Med, Suzhou, Peoples R China.;Univ Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark. (författare)

De två första författarna delar förstaförfattarskapet
Mass spectrometry (MS)-based proteomics workflows typically involve complex, multi-step processes, presenting challenges with sample losses, reproducibility, requiring substantial time and financial investments, and specialized skills. Here we introduce One-Tip, a proteomics methodology that seamlessly integrates efficient, one-pot sample preparation with precise, narrow-window data-independent acquisition (nDIA) analysis. One-Tip substantially simplifies sample processing, enabling the reproducible identification of >9000 proteins from ~1000 HeLa cells. The versatility of One-Tip is highlighted by nDIA identification of ~6000 proteins in single cells from early mouse embryos. Additionally, the study incorporates the Uno Single Cell Dispenser™, demonstrating the capability of One-Tip in single-cell proteomics with >3000 proteins identified per HeLa cell. We also extend One-Tip workflow to analysis of extracellular vesicles (EVs) extracted from blood plasma, demonstrating its high sensitivity by identifying >3000 proteins from 16 ng EV preparation. One-Tip expands capabilities of proteomics, offering greater depth and throughput across a range of sample types.

Sabatier, PierreUppsala universitet,Thoraxkirurgi,Univ Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark(Swepub:uu)piesa271 (författare)
Martin-Gonzalez, JavierUniv Copenhagen, Dept Expt Med, Core Facil Transgenic Mice, Copenhagen, Denmark. (författare)
Eguchi, AkihiroUniv Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark. (författare)
Lechner, MaicoUniv Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark. (författare)
Østergaard, OleUniv Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark. (författare)
Xie, JingshengChinese Acad Med Sci & Peking Union Med Coll, State Key Lab Common Mech Res Major Dis, Suzhou Inst Syst Med, Suzhou, Peoples R China. (författare)
Guo, YuanTecan Grp Ltd, Mannedorf, Switzerland. (författare)
Schultz, LesleyTecan Grp Ltd, Mannedorf, Switzerland. (författare)
Truffer, RafaelaTecan Grp Ltd, Mannedorf, Switzerland. (författare)
Bekker-Jensen, Dorte B.Evosep Biosyst, Odense, Denmark. (författare)
Bache, NicolaiEvosep Biosyst, Odense, Denmark. (författare)
Olsen, Jesper V.Univ Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark. (författare)
Chinese Acad Med Sci & Peking Union Med Coll, State Key Lab Common Mech Res Major Dis, Suzhou Inst Syst Med, Suzhou, Peoples R China.;Univ Copenhagen, Novo Nord Fdn Ctr Prot Res, Copenhagen, Denmark.Thoraxkirurgi (creator_code:org_t)

Av författaren/redakt...: Ye, Zilu; Sabatier, Pierre; Martin-Gonzalez, ...; Eguchi, Akihiro; Lechner, Maico; Østergaard, Ole; visa fler...; Xie, Jingsheng; Guo, Yuan; Schultz, Lesley; Truffer, Rafaela; Bekker-Jensen, D ...; Bache, Nicolai; Olsen, Jesper V.; visa färre...

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