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Inhibition of cysta...
Inhibition of cystathionine-gamma lyase dampens vasoconstriction in mouse and human intracerebral arterioles
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- Peleli, Maria (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Science for Life Laboratory, SciLifeLab,Univ Southern Denmark, Inst Mol Med, Dept Cardiovasc & Renal Res, Odense, Denmark.;Natl & Kapodistrian Univ Athens, Dept Pharm, Lab Pharmacol, Athens, Greece.;Acad Athens, Clin Expt Surg & Translat Res Ctr, Biomed Res Fdn, Athens, Greece.
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- Lyngso, Kristina S. (författare)
- Univ Southern Denmark, Inst Mol Med, Dept Cardiovasc & Renal Res, Odense, Denmark.
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- Poulsen, Frantz Rom (författare)
- Odense Univ Hosp, Dept Neurosurg, Odense, Denmark.;Univ Southern Denmark, Dept Clin Res, Odense, Denmark.;BRIDGE Brain Res Interdisciplinary Guided Excellen, Odense, Denmark.;OPEN Odense Patient Data Explorat Network, Odense, Denmark.
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- Hansen, Pernille B. L. (författare)
- Univ Southern Denmark, Inst Mol Med, Dept Cardiovasc & Renal Res, Odense, Denmark.
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- Papapetropoulos, Andreas (författare)
- Natl & Kapodistrian Univ Athens, Dept Pharm, Lab Pharmacol, Athens, Greece.;Acad Athens, Clin Expt Surg & Translat Res Ctr, Biomed Res Fdn, Athens, Greece.
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- Stubbe, Jane (författare)
- Univ Southern Denmark, Inst Mol Med, Dept Cardiovasc & Renal Res, Odense, Denmark.;Univ Southern Denmark, Inst Mol Med, Dept Cardiovasc & Renal Res, JB Winslows Vej 21,3rd floor, DK-5000 Odense C, Denmark.
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(creator_code:org_t)
- John Wiley & Sons, 2023
- 2023
- Engelska.
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Ingår i: Acta Physiologica. - : John Wiley & Sons. - 1748-1708 .- 1748-1716. ; 239:1
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- AimIn extracerebral vascular beds cystathionine-gamma lyase (CSE) activity plays a vasodilatory role but the role of this hydrogen sulfide (H2S) producing enzyme in the intracerebral arterioles remain poorly understood. We hypothesized a similar function in the intracerebral arterioles. MethodsIntracerebral arterioles were isolated from wild type C57BL/6J mouse (9-12 months old) brains and from human brain biopsies. The function (contractility and secondary dilatation) of the intracerebral arterioles was tested ex vivo by pressure myography using a perfusion set-up. Reverse transcription polymerase chain reaction was used for detecting CSE expression. ResultsCSE is expressed in human and mouse intracerebral arterioles. CSE inhibition with L-propargylglycine (PAG) significantly dampened the K+-induced vasoconstriction in intracerebral arterioles of both species (% of maximum contraction: in human control: 45.4 & PLUSMN; 2.7 versus PAG: 27 & PLUSMN; 5.2 and in mouse control: 50 & PLUSMN; 1.5 versus PAG: 33 & PLUSMN; 5.2) but did not affect the secondary dilatation. This effect of PAG was significantly reversed by the H2S donor sodium hydrosulfide (NaSH) in human (PAG + NaSH: 38.8 & PLUSMN; 7.2) and mouse (PAG + NaSH: 41.7 & PLUSMN; 3.1) arterioles, respectively. The endothelial NO synthase (eNOS) inhibitor, N & omega;-Nitro-l-arginine methyl ester (L-NAME), and the inhibitor of soluble guanylate cyclase (sGC), 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) reversed the effect of PAG on the K+-induced vasoconstriction in the mouse arterioles and attenuated the K+-induced secondary dilatation significantly. ConclusionCSE contributes to the K+-induced vasoconstriction via a mechanism involving H2S, eNOS, and sGC whereas the secondary dilatation is regulated by eNOS and sGC but not by CSE.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Nyckelord
- contractility
- cystathionine-gamma lyase
- endothelial NO synthase
- hydrogen sulfide
- intracerebral arterioles
- soluble guanylate cyclase
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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