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A quality by design...
A quality by design approach to optimise disulfide-linked hyaluronic acid hydrogels
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- Ranamalla, Saketh Reddy (författare)
- Babes Bolyai Univ, Fac Biol & Geol, Doctoral Sch Integrat Biol, Cluj Napoca 400015, Romania.;Univ Med & Pharm Iuliu Ha?ieganu, Fac Pharm, Dept Pharmaceut Technol & Biopharm, Cluj Napoca 400010, Romania.
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- Tavakoli, Shima (författare)
- Uppsala universitet,Makromolekylär kemi
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- Porfire, Alina Silvia (författare)
- Univ Med & Pharm Iuliu Ha?ieganu, Fac Pharm, Dept Pharmaceut Technol & Biopharm, Cluj Napoca 400010, Romania.
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- Tefas, Lucia Ruxandra (författare)
- Univ Med & Pharm Iuliu Ha?ieganu, Fac Pharm, Dept Pharmaceut Technol & Biopharm, Cluj Napoca 400010, Romania.
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- Banciu, Manuela (författare)
- Babe? Bolyai Univ, Fac Biol & Geol, Ctr Syst Biol Biodivers & Bioresources, Dept Mol Biol & Biotechnol, Cluj Napoca 400015, Romania.
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- Tomuța, Ioan (författare)
- Univ Med & Pharm Iuliu Ha?ieganu, Fac Pharm, Dept Pharmaceut Technol & Biopharm, Cluj Napoca 400010, Romania.
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- Varghese, Oommen P., 1977- (författare)
- Uppsala universitet,Makromolekylär kemi
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Babes Bolyai Univ, Fac Biol & Geol, Doctoral Sch Integrat Biol, Cluj Napoca 400015, Romania;Univ Med & Pharm Iuliu Ha?ieganu, Fac Pharm, Dept Pharmaceut Technol & Biopharm, Cluj Napoca 400010, Romania. Makromolekylär kemi (creator_code:org_t)
- Elsevier, 2024
- 2024
- Engelska.
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Ingår i: Carbohydrate Polymers. - : Elsevier. - 0144-8617 .- 1879-1344. ; 339
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- n this study, the disulfide-linked hyaluronic acid (HA) hydrogels were optimised for potential application as a scaffold in tissue engineering through the Quality by Design (QbD) approach. For this purpose, HA was first modified by incorporating the cysteine moiety into the HA backbone, which promoted the formation of disulfide cross-linked HA hydrogel at physiological pH. Utilising a Design of Experiments (DoE) methodology, the critical factors to achieve stable biomaterials, i.e. the degree of HA substitution, HA molecular weight, and coupling agent ratio, were explored. To establish a design space, the DoE was performed with 65 kDa, 138 kDa and 200 kDa HA and variable concentrations of coupling agent to optimise conditions to obtain HA hydrogel with improved rheological properties. Thus, HA hydrogel with a 12 % degree of modification, storage modulus of ≈2321 Pa and loss modulus of ≈15 Pa, was achieved with the optimum ratio of coupling agent. Furthermore, biocompatibility assessments in C28/I2 chondrocyte cells demonstrated the non-toxic nature of the hydrogel, underscoring its potential for tissue regeneration. Our findings highlight the efficacy of the QbD approach in designing HA hydrogels with tailored properties for biomedical applications.
Ämnesord
- NATURVETENSKAP -- Kemi -- Polymerkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Polymer Chemistry (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Kemiteknik -- Polymerteknologi (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Chemical Engineering -- Polymer Technologies (hsv//eng)
Nyckelord
- Hyaluronic acid
- Hydrogels
- Quality by design
- Failure mode and effects analysis
- Design of Experiments
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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