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  • Sych, TarasKarolinska Inst, Dept Womens & Childrens Hlth, Sci Life Lab, Solna, Sweden. (author)

High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler

  • Article/chapterEnglish2024

Publisher, publication year, extent ...

  • Nature Publishing Group,2024
  • electronicrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-533239
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-533239URI
  • https://doi.org/10.1038/s41587-023-01825-5DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:152959758URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Lipid nanoparticles, viruses, exosomes and liposomes are characterized by analysis of fluorescence fluctuations. We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5-200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Schlegel, JanKarolinska Inst, Dept Womens & Childrens Hlth, Sci Life Lab, Solna, Sweden. (author)
  • Barriga, Hanna M. G.Karolinska Institutet,Karolinska Inst, Dept Med Biochem & Biophys, Stockholm, Sweden. (author)
  • Ojansivu, MiinaKarolinska Institutet,Karolinska Inst, Dept Med Biochem & Biophys, Stockholm, Sweden. (author)
  • Hanke, LeoKarolinska Institutet,Karolinska Inst, Div Infect Dis, Dept Med Solna, Stockholm, Sweden.;Karolinska Inst, Ctr Mol Med, Stockholm, Sweden. (author)
  • Weber, FlorianKarolinska Inst, Dept Womens & Childrens Hlth, Sci Life Lab, Solna, Sweden.;Univ Appl Sci Upper Austria, Dept Med Engn, Linz, Austria. (author)
  • Bostancioglu, R. BeklemKarolinska Inst, Dept Lab Med, Huddinge, Sweden. (author)
  • Ezzat, KariemKarolinska Inst, Dept Lab Med, Huddinge, Sweden. (author)
  • Stangl, HerbertMed Univ Vienna, Inst Med Chem, Ctr Pathobiochem & Genet, Vienna, Austria. (author)
  • Plochberger, BirgitUniv Appl Sci Upper Austria, Dept Med Engn, Linz, Austria.;LBG Ludwig Boltzmann Inst Traumatol, Nanoscopy, Vienna, Austria. (author)
  • Laurencikiene, JurgaKarolinska Institutet,Karolinska Inst, Dept Med Huddinge, Lipid Lab, Stockholm, Sweden. (author)
  • El Andaloussi, SamirKarolinska Institutet,Karolinska Inst, Dept Lab Med, Huddinge, Sweden. (author)
  • Fuerth, DanielUppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)danfu839 (author)
  • Stevens, Molly M.Karolinska Institutet,Karolinska Inst, Dept Med Biochem & Biophys, Stockholm, Sweden.;Imperial Coll London, Dept Mat, Dept Bioengn, London, England.;Imperial Coll London, Inst Biomed Engn, London, England. (author)
  • Sezgin, ErdincKarolinska Institutet,Karolinska Inst, Dept Womens & Childrens Hlth, Sci Life Lab, Solna, Sweden. (author)
  • Karolinska InstitutetKarolinska Inst, Dept Womens & Childrens Hlth, Sci Life Lab, Solna, Sweden. (creator_code:org_t)

Related titles

  • In:Nature Biotechnology: Nature Publishing Group42:41087-01561546-1696

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