Development of Enhanced Analytical Methodology for Lipid Analysis from Sampling to Detection: A Targeted Lipidomics Approach

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Isaac, Giorgis,1974-Uppsala universitet,Avdelningen för analytisk kemi (författare)

Development of Enhanced Analytical Methodology for Lipid Analysis from Sampling to Detection : A Targeted Lipidomics Approach

BokEngelska2005

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Uppsala :Acta Universitatis Upsaliensis,2005
48 s.
electronicrdacarrier

Nummerbeteckningar

LIBRIS-ID:oai:DiVA.org:uu-5810
ISBN:9155462596
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5810URI

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Språk:engelska
Sammanfattning på:engelska

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Ämneskategori:dok swepub-publicationtype

Serie

Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology,1651-6214 ;56

Anmärkningar

This thesis covers a wide range of analytical method development for lipid analysis in complex biological samples; from sample preparation using pressurized fluid extraction (PFE) and separation with reversed phase capillary liquid chromatography (RP-LC) to detection by electrospray ionization mass spectrometry (ESI/MS) and tandem MS.The requirements for fast, reliable and selective extraction methods with minimal usage of solvents have accelerated the development of new extraction techniques. PFE is one of the new automated, fast and efficient liquid extraction techniques which use elevated temperature and pressure with standard liquid solvents. In this thesis the reliability and efficiency of the PFE technique was investigated for the extraction of total lipid content from cod, herring muscle and human brain tissue as well as for pesticides from fatty foodstuffs. Improved or comparable efficiencies were achieved with reduced time and solvent consumption as compared to traditional methods. A RP-LC coupled online to ESI/MS for the analysis of phosphatidylcholine (PC) and sphingomyelin (SM) molecular species was developed and used for the analysis of brain lipids from eight groups of mice treated with vehicle and various neuroleptics. The effect of postnatal iron administration in lipid composition and behavior was investigated. Whether or not these effects could be altered by subchronic administration of the neuroleptics (clozapine and haloperidol) were examined. The results support the hypothesis that an association between psychiatric disorders, behavior abnormalities and lipid membrane constitution in the brain exists.Finally, a tandem MS precursor ion scan was used to analyze the developmental profile of brain sulfatide accumulation in arylsulfatase A (ASA) deficient (ASA -/-) as compared to wild type control (ASA +/+) mice. The ASA -/- mice were developed as a model of the monogenic disease metachromatic leukodystrophy with an established deficiency of the lysosomal enzyme ASA. The results showed that an alteration in the composition of sulfatide molecular species was observed between the ASA -/- and ASA +/+ mice.This thesis shows that modern analytical methods can provide new insights in the extraction and analysis of lipids from complex biological samples.

Ämnesord och genrebeteckningar

NATURVETENSKAP Kemi Analytisk kemi hsv//swe
NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
Analytical chemistry
electrospray ionization
lipids
liquid chromatography
mass spectrometry
pesticides
phosphatidylcholine
pressurized fluid extraction
sphingomyelin
sulfatides
Analytisk kemi
Analytical chemistry
Analytisk kemi

Biuppslag (personer, institutioner, konferenser, titlar ...)

Bergquist, Jonas (preses)
Markides, Karin (preses)
Waldebäck, Monica (preses)
Christie, William,PhDLipid Analysis Unit, Scottish Crop Research Institute, Dundee (opponent)
Uppsala universitetAvdelningen för analytisk kemi (creator_code:org_t)

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Av författaren/redakt...: Isaac, Giorgis, ...; Bergquist, Jonas; Markides, Karin; Waldebäck, Monic ...; Christie, Willia ...

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NATURVETENSKAP: NATURVETENSKAP; och Kemi; och Analytisk kemi

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Av lärosätet: Uppsala universitet

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