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- Dahlin, Andreas,1976-Uppsala universitet,Kemiska institutionen (author)
Microscale Tools for Sample Preparation, Separation and Detection of Neuropeptides
- BookEnglish2005
Publisher, publication year, extent ...
- Uppsala :Kemiska institutionen,2005
- 62 s.
- electronicrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:uu-5838
- ISBN:9155462790
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5838URI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Varying forms of title
- Mikroskaliga verktyg för provpreparering, separation och detektion av neuropeptider
Series
- Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology,1651-6214 ;64
Notes
- The analysis of low abundant biological molecules is often challenging due to their chemical properties, low concentration and limited sample volumes. Neuropeptides are one group of molecules that fits these criteria. Neuropeptides also play an important role in biological functions, which makes them extra interesting to analyze. A classic chemical analysis involves sampling, sample preparation, separation and detection. In this thesis, an enhanced solid supported microdialysis method was developed and used as a combined sampling- and preparation technique. In general, significantly increased extraction efficiency was obtained for all studied peptides. To be able to control the small sample volumes and to minimize the loss of neuropeptides because of unwanted adsorption onto surfaces, the subsequent analysis steps were miniaturized to a micro total analysis system (µ-TAS), which allowed sample pre-treatment, injection, separation, manipulation and detection. In order to incorporate these analysis functions to a microchip, a novel microfabrication protocol was developed. This method facilitated three-dimensional structures to be fabricated without the need of clean room facilities. The sample pre-treatment step was carried out by solid phase extraction from beads packed in the microchip. Femtomole levels of neuropeptides were detected from samples possessing the same properties as microdialysates. The developed injection system made it possible to conduct injections from a liquid chromatographic separation into a capillary electrophoresis channel, which facilitated for advanced multidimensional separations. An electrochemical sample manipulation system was also developed. In the last part, different electrospray emitter tip designs made directly from the edge of the microchip substrate were developed and evaluated. The emitters were proven to be comparable with conventional, capillary based emitters in stability, durability and dynamic flow range. Although additional developments remain, the analysis steps described in this thesis open a door to an integrated, on-line µ-TAS for neuropeptides analysis in complex biological samples.
Subject headings and genre
- NATURVETENSKAP Kemi Analytisk kemi hsv//swe
- NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
- Analytical chemistry
- Neuropeptides
- Microchip
- Enhanced microdialysis
- Poly(dimethylsiloxane) (PDMS)
- Electrospray ionization (ESI)
- Multidimensional separation
- Electrochemical manipulation
- Mass spectrometry (MS)
- Capillary electrophoresis (CE)
- Microdevice
- Microfabrication
- Micro total analysis system (μ-TAS)
- Analytisk kemi
- Analytical chemistry
- Analytisk kemi
Added entries (persons, corporate bodies, meetings, titles ...)
- Bergquist, Jonas (thesis advisor)
- Markides, Karin E. (thesis advisor)
- Kennedy, Robert T. (opponent)
- Uppsala universitetKemiska institutionen (creator_code:org_t)
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