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Ex vivo stimulation...
Ex vivo stimulation of cytomegalovirus (CMV)-specific T cells using CMVpp65-modified dendritic cells as stimulators
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- Carlsson, Björn (författare)
- Uppsala universitet,Enheten för klinisk immunologi
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- Cheng, Wing-Shing (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi
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- Tötterman, Thomas H. (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi
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- Essand, Magnus (författare)
- Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi
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(creator_code:org_t)
- 2003-04-28
- 2003
- Engelska.
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Ingår i: British Journal of Haematology. - : Wiley. - 0007-1048 .- 1365-2141. ; 121:3, s. 428-38
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Cytomegalovirus (CMV) infection is a dangerous complication in immunosuppressed individuals such as allogeneic stem cell transplant patients. CMV disease can be prevented by the early post-transplant transfer of donor-derived, CMV-directed, T cells. Fast and cost efficient methods to generate CMV-specific T cells are, therefore, warranted. The current study utilized peptide-pulsed and adenovirus-transduced dendritic cells (DC) to generate CMV-restricted T cells. After one stimulation with CMV pp65495-503 peptide-pulsed DC and three re-stimulations with peptide-pulsed monocytes, virtually all T cells were CD8+, expressed the relevant T cell receptor and exhibited high peptide-specific lytic activity. After only one stimulation, pp65495-503-restricted T cells could be sorted to a purity of higher than 95% and expanded up to 1000-fold in 2 weeks. This technique may prove useful for the rapid generation of large quantities of specific cytolytic T lymphocytes (CTL) for cell therapy. DC transduced with an adenoviral vector encoding the full-length pp65 protein (Adpp65) were able to simultaneously expand CTL against multiple epitopes of pp65. In addition, they activated CMV-specific CD4+ T-helper cells. This approach would stimulate multiple-epitope populations of pp65-specific T cells and could be made available to patients of any human leucocyte antigen (HLA) haplotype. DC transduced with adenoviral vectors to express full-length antigens may prove to be potent vaccines against viral pathogens and cancer.
Nyckelord
- cytomegalovirus
- pp65
- dendritic cells
- T-cell expansion
- tetramers
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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