Precursor of human adenovirus core polypeptide Mu targets the nucleolus and modulates the expression of E2 proteins

• We have examined the subcellular localization properties of human adenovirus 2 (HAdV-2) preMu and mature Mu (pX) proteins as fusions with enhanced green fluorescence protein (EGFP). We determined that preMu is exclusively a nucleolar protein with a single nucleolar accumulation signal within the Mu sequence. In addition, we noted that both preMu-EGFP and Mu-EGFP are excluded from adenovirus DNA-binding protein (DBP)-rich replication centres in adenovirus-infected cells. Surprisingly, we observed that cells in which preMu-EGFP (but not Mu-EGFP) is transiently expressed prior to or shortly after infection with Ad2 did not express late adenovirus genes. Further investigation suggested this might be due to a failure to express pre-terminal protein (preTP) from the E2 region, despite expression of another E2 protein, DBP. Deletion mutagenesis identified a highly conserved region in the C terminus of preMu responsible for these observations. Thus our data suggest that preMu may play a role in modulating accumulation of proteins from the E2 region.

Nyckelord

Adenovirus E2 Proteins/*metabolism

Adenoviruses; Human/pathogenicity

Amino Acid Sequence

Cell Nucleolus/*metabolism

DNA-Binding Proteins/*metabolism

Gene Deletion

Gene Expression Regulation; Viral

Green Fluorescent Proteins

Hela Cells

Humans

Luminescent Proteins/genetics/metabolism

Molecular Sequence Data

Peptides/*metabolism

Protein Precursors/*metabolism

Recombinant Fusion Proteins/metabolism

Research Support; Non-U.S. Gov't

Viral Core Proteins/*metabolism

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