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Autoantibodies agai...
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Boe, Anette S.Uppsala universitet,Institutionen för medicinska vetenskaper,Autoimmuna sjukdomar (Kämpe)
(author)
Autoantibodies against 21-hydroxylase and side-chain cleavage enzyme in autoimmune Addison's disease are mainly immunoglobulin G1
- Article/chapterEnglish2004
Publisher, publication year, extent ...
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Oxford University Press (OUP),2004
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-72169
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-72169URI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:19895662URI
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https://doi.org/10.1530/eje.0.1500049DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:vet swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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OBJECTIVE: Immunoglobulin G (IgG) antibodies to the steroidogenic enzymes 21-hydroxylase (21OH) and side-chain cleavage enzyme (SCC) are important diagnostic markers for autoimmune Addison's disease and autoimmune polyendocrine syndromes (APS) types I and II. The characterization of autoantibody (IgG) subclasses may reveal information on how tIssue destruction takes place; therefore, IgG subtypes of anti-21OH and anti-SCC antibodies from sera of patients with Addison's disease, APS I and APS II were determined using recombinant 21OH and SCC. METHODS: SCC(51-521) and his-SCC(51-521) were expressed by pET-scc in the Escherichia coli strain BL21 Star (DE3) and inclusion bodies were purified. Full-length, human 21OH fused to an N-terminal 6x histidine affinity tag was expressed in insect cells by using the baculovirus expression system bac-to-bac. Western blots were used to investigate the IgG subtype(s) of the autoantibodies against 21OH and SCC in patients and healthy blood donors. RESULTS: All anti-SCC positive sera (n=10) contained autoantibodies of the IgG1 subclass, while four out of ten also contained IgG3. All anti-21OH positive sera (n=16) had autoantibodies exclusively against IgG1. Sera from 20 healthy subjects did not show any reactivity against 21OH or SCC. CONCLUSIONS: The finding of a predominating IgG1 response against 21OH and SCC may suggest that T helper (Th) cells of the Th1 subclass are involved in destruction of the adrenal cortex in patients with autoimmune Addison's disease.
Subject headings and genre
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Addison Disease/*immunology
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Antibody Specificity
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Autoantibodies/blood/*immunology
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Biological Markers
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Cholesterol Side-Chain Cleavage Enzyme/genetics/*immunology
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Gene Expression Regulation; Enzymologic
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Humans
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Immunoglobulin G/blood/immunology
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Research Support; Non-U.S. Gov't
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Steroid 21-Hydroxylase/genetics/*immunology
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T-Lymphocytes/immunology
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MEDICINE
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MEDICIN
Added entries (persons, corporate bodies, meetings, titles ...)
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Bredholt, Geir
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Knappskog, Per M.
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Hjelmervik, Trond Ove
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Mellgren, Gunnar
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Winqvist, OlaKarolinska Institutet,Uppsala universitet,Institutionen för medicinska vetenskaper,Autoimmuna sjukdomar (Kämpe)
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Kämpe, OlleUppsala universitet,Institutionen för medicinska vetenskaper,Autoimmuna sjukdomar (Kämpe)(Swepub:uu)ollekamp
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Husebye, Eystein S.
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Uppsala universitetInstitutionen för medicinska vetenskaper
(creator_code:org_t)
Related titles
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In:European Journal of Endocrinology: Oxford University Press (OUP)150:1, s. 49-560804-46431479-683X
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