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  • Boe, Anette S.Uppsala universitet,Institutionen för medicinska vetenskaper,Autoimmuna sjukdomar (Kämpe) (author)

Autoantibodies against 21-hydroxylase and side-chain cleavage enzyme in autoimmune Addison's disease are mainly immunoglobulin G1

  • Article/chapterEnglish2004

Publisher, publication year, extent ...

  • Oxford University Press (OUP),2004
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-72169
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-72169URI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:19895662URI
  • https://doi.org/10.1530/eje.0.1500049DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:vet swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • OBJECTIVE: Immunoglobulin G (IgG) antibodies to the steroidogenic enzymes 21-hydroxylase (21OH) and side-chain cleavage enzyme (SCC) are important diagnostic markers for autoimmune Addison's disease and autoimmune polyendocrine syndromes (APS) types I and II. The characterization of autoantibody (IgG) subclasses may reveal information on how tIssue destruction takes place; therefore, IgG subtypes of anti-21OH and anti-SCC antibodies from sera of patients with Addison's disease, APS I and APS II were determined using recombinant 21OH and SCC. METHODS: SCC(51-521) and his-SCC(51-521) were expressed by pET-scc in the Escherichia coli strain BL21 Star (DE3) and inclusion bodies were purified. Full-length, human 21OH fused to an N-terminal 6x histidine affinity tag was expressed in insect cells by using the baculovirus expression system bac-to-bac. Western blots were used to investigate the IgG subtype(s) of the autoantibodies against 21OH and SCC in patients and healthy blood donors. RESULTS: All anti-SCC positive sera (n=10) contained autoantibodies of the IgG1 subclass, while four out of ten also contained IgG3. All anti-21OH positive sera (n=16) had autoantibodies exclusively against IgG1. Sera from 20 healthy subjects did not show any reactivity against 21OH or SCC. CONCLUSIONS: The finding of a predominating IgG1 response against 21OH and SCC may suggest that T helper (Th) cells of the Th1 subclass are involved in destruction of the adrenal cortex in patients with autoimmune Addison's disease.

Subject headings and genre

  • Addison Disease/*immunology
  • Antibody Specificity
  • Autoantibodies/blood/*immunology
  • Biological Markers
  • Cholesterol Side-Chain Cleavage Enzyme/genetics/*immunology
  • Gene Expression Regulation; Enzymologic
  • Humans
  • Immunoglobulin G/blood/immunology
  • Research Support; Non-U.S. Gov't
  • Steroid 21-Hydroxylase/genetics/*immunology
  • T-Lymphocytes/immunology
  • MEDICINE
  • MEDICIN

Added entries (persons, corporate bodies, meetings, titles ...)

  • Bredholt, Geir (author)
  • Knappskog, Per M. (author)
  • Hjelmervik, Trond Ove (author)
  • Mellgren, Gunnar (author)
  • Winqvist, OlaKarolinska Institutet,Uppsala universitet,Institutionen för medicinska vetenskaper,Autoimmuna sjukdomar (Kämpe) (author)
  • Kämpe, OlleUppsala universitet,Institutionen för medicinska vetenskaper,Autoimmuna sjukdomar (Kämpe)(Swepub:uu)ollekamp (author)
  • Husebye, Eystein S. (author)
  • Uppsala universitetInstitutionen för medicinska vetenskaper (creator_code:org_t)

Related titles

  • In:European Journal of Endocrinology: Oxford University Press (OUP)150:1, s. 49-560804-46431479-683X

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