Chromatin from two classes of platyhelminthes display both protist H1 and higher eukaryote core histones

• Histones from the parasitic platyhelminthes, Echinococcus granulosus and Fasciola hepatica, were systematically characterized. Core histones H2A, H2B, H3 and H4, which were identified on the basis of amino acid sequencing and mass spectrometry data, showed conserved electrophoretic patterns. Histones H1, identified on the basis of physicochemical properties, amino acid composition and amino acid sequencing, showed divergence, both in their number and electrophoretic mobilities, between the two species and among other organisms. According to these data, core histones but not H1 histones, would be stabilized during evolution at the level of platyhelminthes.

Nyckelord

Amino Acid Sequence

Amino Acids/analysis/chemistry/metabolism

Animals

Cattle

Chromatin/*chemistry/metabolism

Chromatography; High Pressure Liquid

Echinococcus/*chemistry/metabolism

Electrophoresis; Polyacrylamide Gel

Fasciola hepatica/*chemistry/metabolism

Histones/*chemistry/genetics/*isolation & purification/metabolism

Research Support; Non-U.S. Gov't

Sea Urchins/chemistry/metabolism

Spectrometry; Mass; Matrix-Assisted Laser Desorption-Ionization

Thymus Gland/chemistry/metabolism

Trypanosoma cruzi/chemistry/metabolism

Publikations- och innehållstyp

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