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Induction of apopto...
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Li, LiUppsala universitet,Institutionen för kvinnors och barns hälsa,Klinisk och experimentell reproduktionsbiologi/Olovsson
(author)
Induction of apoptosis and G2/M arrest by 2-methoxyestradiol in human cervical cancer HeLaS3 cells.
- Article/chapterEnglish2004
Publisher, publication year, extent ...
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Athens :International institute for anticancer research,2004
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-73059
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-73059URI
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-122172URI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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BACKGROUND: It has been demonstrated that 2-Methoxyestradiol (2-ME), one of the estrogen metabolites, induces apoptosis in many different tumor cell lines. In the present study, the effects of 2-ME on human cervical cancer HeLaS3 cells and on normal cervical epithelial cells were evaluated. MATERIALS AND METHODS: Acridine orange staining, DNA fragmentation arrays and flow cytometry were used to measure the apoptosis and cell cycle progression. In addition, the effect of 2-ME on expression of iNOS was measured by Western blot. RESULTS: 2-ME inhibited the growth of HeLaS3 cells. This growth inhibition was accompanied by apoptosis and G2/M cell cycle arrest. 2-ME increased the expression of iNOS in parallel with apoptosis. Moreover, apoptosis was prevented by the iNOS inhibitor 1400W. 2-ME treatment resulted in a slight increase of the G2/M population, but no apoptosis, in normal cervical epithelial cells. There was no synergetic effect between E2 and 2-ME. CONCLUSION: 2-ME induced apoptosis via the iNOS pathway and caused G2/M cell cycle arrest in human cervical cancer HeLaS3 cells, but showed only slight effects on normal cervical epithelial cells. These data suggest that 2-ME might be an adjuvant agent in the treatment of cervical cancer.
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Added entries (persons, corporate bodies, meetings, titles ...)
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Bu, ShizhongLudwiginstitutet för Cancerforskning
(author)
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Bäckström, TorbjörnUmeå universitet,Obstetrik och gynekologi(Swepub:umu)toba0001
(author)
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Landström, MaréneLudwiginstitutet för Cancerforskning(Swepub:uu)mareland
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Ulmsten, Ulf
(author)
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Fu, XinUppsala universitet,Institutionen för kvinnors och barns hälsa,Klinisk och experimentell reproduktionsbiologi/Olovsson(Swepub:uu)faxinfu
(author)
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Uppsala universitetInstitutionen för kvinnors och barns hälsa
(creator_code:org_t)
Related titles
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In:Anticancer ResAthens : International institute for anticancer research24:2B, s. 873-800250-70051791-7530
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