Digital image processing for multiplexing of single molecule detection

Pinidiyaarachchi, AmalkaUppsala universitet,Centrum för bildanalys,Datoriserad bildanalys (author)

Details and applications of the single molecule detection were presented by Mats Nilsson during the session on "Föredrag forskning III, Varia".
Using padlock and proximity probing techniques, individual molecular identification events are converted to long DNA molecules, carrying repeated sequence motifs used for identification of the detected molecules. We show that identification events can be amplified using rolling circle replication, and randomly attached to a surface for repeated access by identification probes. Repeated hybridization with detection probes carrying fluorescing nano-crystals (quantum dots) of varying spectral properties opens the possibility to search for large numbers of different identification events simultaneously. Methods for digital image processing of the resulting multi-spectral data include spatial as well as spectral data clustering. Spatial data processing includes registration of images from repeated hybridization events as well as delineation of clustered reporter events. Spectral data processing and analysis includes classification of spectral data into groups of either pre-defined or unknown patterns representing different molecular identification events.

Göransson, JennyUppsala universitet,Institutionen för genetik och patologi(Swepub:uu)jegor108 (author)
Gonzalez-Rey, CarlosUppsala universitet,Institutionen för genetik och patologi(Swepub:uu)cagre677 (author)
Howell, MathiasUppsala universitet,Institutionen för genetik och patologi(Swepub:uu)mathhowe (author)
Melin, JonasUppsala universitet,Institutionen för teknikvetenskaper(Swepub:uu)jomel499 (author)
Jarvius, JonasUppsala universitet,Institutionen för genetik och patologi(Swepub:uu)jonajarv (author)
Nilsson, MatsUppsala universitet,Institutionen för genetik och patologi(Swepub:uu)matsnils (author)
Bengtsson, EwertUppsala universitet,Centrum för bildanalys(Swepub:uu)ewertbs (author)
Wählby, CarolinaUppsala universitet,Centrum för bildanalys,Institutionen för genetik och patologi(Swepub:uu)cli05194 (author)
Uppsala universitetCentrum för bildanalys (creator_code:org_t)

By the author/editor: Pinidiyaarachchi ...; Göransson, Jenny; Gonzalez-Rey, Ca ...; Howell, Mathias; Melin, Jonas; Jarvius, Jonas; show more...; Nilsson, Mats; Bengtsson, Ewert; Wählby, Carolina; show less...

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