Sökning: L773:1064 3745 OR L773:1940 6029 OR L773:9781071613986 >
Universal tag array...
Universal tag arrays in forensic SNP analysis.
-
- Allen, Marie (författare)
- Uppsala universitet,Molekylärbiologi,Institutionen för immunologi, genetik och patologi
-
- Divne, Anna-Maria (författare)
- Uppsala universitet,Molekylärbiologi,Institutionen för immunologi, genetik och patologi
-
(creator_code:org_t)
- 2005
- 2005
- Engelska.
-
Ingår i: Methods in Molecular Biology. - 1064-3745 .- 1940-6029. ; 297, s. 141-154
- Relaterad länk:
-
http://www.ncbi.nlm....
-
visa fler...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- Microarray-based single nucleotide polymorphism (SNP) genotyping enables simultaneous and rapid detection of a large number of markers and is thus an attractive method for forensic individual acid identification. This assay relies on a one-color detection system and minisequencing in solution before hybridization to universal tag arrays. The minisequencing reaction is based on incorporation of a fluorescent dideoxynucleotide to a primer containing a tag-sequence flanking the position to be interrogated. This one-color system detects C and T polymorphisms in separate reactions on multiple polymerase chain reaction targets with the fluorophore TAMRA coupled to the respective dideoxynucleotide. After incorporation, tagged primer sequences are hybridized through their complementary sequence on the array, and positive signals are detected by a confocal laser-scanner.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Annan medicin och hälsovetenskap -- Rättsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Other Medical and Health Sciences -- Forensic Science (hsv//eng)
Nyckelord
- Base sequence
- DNA primers
- forensic medicine
- humans
- nucleic acid hybridization
- polymerase chain reaction
- polymorphism
- single nucleotide
- research support
- non-U.S. Gov't
- microarrays
- universal tag-arrays
- minisequencing
- forensic
- SNP
- Forensic genetics
- Rättsgenetik
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas