Cellobiohydrolase I from Trichoderma reesei: Identification of an active-site nucleophile and additional information on sequence including the glycosylation pattern of the core protein

• (R,S)-3,4-Epoxybutyl beta-cellobioside, but not the corresponding propyl and pentyl derivatives, inactivates specifically and irreversibly cellobiohydrolase I from Trichoderma reesei by covalent modification of Glu(212), the putative active-site nucleophi

Nyckelord

cellobiohydrolase I; Trichoderma reesei; epoxybutyl cellobioside; active-site nucleophile; electrospray ionization mass spectrometry; MASS-SPECTROMETRY; LIMITED PROTEOLYSIS; MOLECULAR-CLONING; HYDROLASES; 4-GLUCANOHYDROLASE; CLASSIFICATION; SIMILARITIES

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