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  • Lexander, Helena (author)

Correlation of protein expression, Gleason score and DNA ploidy in prostate cancer

  • Article/chapterEnglish2006

Publisher, publication year, extent ...

  • Wiley,2006
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-81666
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-81666URI
  • https://doi.org/10.1002/pmic.200600148DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:1932626URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • The prognosis of prostate cancer correlates with tumor differentiation. Gleason score and DNA ploidy are two prognostic factors that correlate with prognosis. We analyzed differences in protein expression in prostate cancer of high and low aggressiveness according to these measures. From 35 prostatectomy specimens, 29 cancer samples and 10 benign samples were harvested by scraping cells from cut surfaces. DNA ploidy was assessed by image cytometry. Protein preparations from cell suspensions were examined by 2-DE. Protein spots that differed quantitatively between sample groups were identified by MS fingerprinting of tryptic fragments and MS/MS sequence analysis. We found 39 protein spots with expression levels that were raised or lowered in correlation with Gleason score and/or DNA ploidy pattern (31 overexpressed in high-malignant cancer, 8 underexpressed). Of these, 30 were identified by MS. Among overexpressed proteins were heat-shock, structural and membrane proteins and enzymes involved in gene silencing, protein synthesis/degradation, mitochondrial protein import (metaxin 2), detoxification (GST-pi) and energy metabolism. Stroma-associated proteins were generally underexpressed. The protein expression of prostate cancer correlates with tumor differentiation. Potential prognostic markers may be found among proteins that are differentially expressed and the clinical value of these should be validated.

Subject headings and genre

  • 2-DE
  • DNA ploidy
  • Gleason score
  • prognosis
  • prostate cancer
  • MEDICINE
  • MEDICIN

Added entries (persons, corporate bodies, meetings, titles ...)

  • Palmberg, CarinaKarolinska Institutet (author)
  • Hellman, UlfUppsala universitet,Ludwiginstitutet för cancerforskning(Swepub:uu)ulfhm (author)
  • Auer, GertKarolinska Institutet (author)
  • Hellström, MagnusKarolinska Institutet (author)
  • Franzén, BoKarolinska Institutet (author)
  • Jörnvall, HansKarolinska Institutet (author)
  • Egevad, LarsKarolinska Institutet (author)
  • Karolinska InstitutetLudwiginstitutet för cancerforskning (creator_code:org_t)

Related titles

  • In:Proteomics: Wiley6:15, s. 4370-43801615-98531615-9861

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  • Proteomics (Search for host publication in LIBRIS)

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