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  • Steinmüller-Nethl, Doris (author)

Strong binding of bioactive BMP-2 to nanocrystalline diamond by physisorption

  • Article/chapterEnglish2006

Publisher, publication year, extent ...

  • Elsevier BV,2006
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-82100
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-82100URI
  • https://doi.org/10.1016/j.biomaterials.2006.04.036DOI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Nano-crystalline diamond (NCD)-coated surfaces were efficiently functionalized with bone morphogenetic protein-2 (BMP-2) by means of physisorption. Due to their randomly oriented texture, NCD-coated surfaces appear to bind complex molecules firmly. Applying various highly sensitive analytical methods, the interaction was found extremely stable. The strength of the experimentally measured adherence between BMP-2 and NCD was further corroborated by theoretical calculations. Oxygen treatment rendered NCD hydrophilic by the appearance of surface oxygen containing groups. This particular NCD surface exhibited even higher binding energies towards BMP-2 than the hydrophobic surface, and this surface was also favoured by cultured cells. Most importantly in this context, bound BMP-2 was found fully active. When cultured on BMP-2-treated NCD, osteosarcoma cells strongly up-regulated alkaline phosphatase, a specific marker for osteogenic differentiation. Hence, this simple method will allow generating highly versatile surfaces with complex biomimetic coatings, essentials for novel medical devices and implants as well as for innovative scaffolds in tissue engineering.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Kloss, Frank (author)
  • Najam-Ul-Haq, Muhammed (author)
  • Rainer, Matthias (author)
  • Larsson, KarinUppsala universitet,Institutionen för materialkemi,Oorganisk kemi,oorganisk kemi(Swepub:uu)karinl (author)
  • Linsmeier, Christian (author)
  • Köhler, Gottfried (author)
  • Fehrer, Christine (author)
  • Lepperdinger, Günter (author)
  • Liu, Xianjie (author)
  • Memmel, Norbert (author)
  • Bertel, Erminald (author)
  • Huck, Christian W. (author)
  • Gassner, Robert (author)
  • Bonn, Günther (author)
  • Uppsala universitetInstitutionen för materialkemi (creator_code:org_t)

Related titles

  • In:Biomaterials: Elsevier BV27, s. 4547-45560142-9612

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