Interaction between Escherichia coli RNase P RNA and the discriminator base results in slow product release

• We suggested previously that a purine at the discriminator base position ina tRNA precursor interacts with the well-conserved U294 in M1 RNA, the catalytic subunit of Escherichia coli RNase P. Here we investigated this interaction and its influence on the

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catalytic RNA; enzyme-substrate recognition; ribozyme; RNA kinetics; tRNA identity; RIBONUCLEASE-P; CATALYTIC SUBUNIT; M1 RNA; MAGNESIUM-IONS; CLEAVAGE SITE; ENZYME; PRECURSORS; SEQUENCES; MOIETY

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